Reference - Detail
|Author||Kagoshima H, Nimmo R, Saad N, Tanaka J, Miwa Y, Mitani S, Kohara Y, Woollard A.|
|Title||The C. elegans CBFbeta homologue BRO-1 interacts with the Runx factor, RNT-1, to promote stem cell proliferation and self-renewal.|
In this report, we investigate the C. elegans CBFbeta homologue, BRO-1. bro-1 mutants have a similar male-specific sensory ray loss phenotype to rnt-1 (the C. elegans homologue of the mammalian CBFbeta-interacting Runx factors), caused by failed cell divisions in the seam lineages. Our studies indicate that BRO-1 and RNT-1 form a cell proliferation-promoting complex, and that BRO-1 increases both the affinity and specificity of RNT-1-DNA interactions. Overexpression of bro-1, like rnt-1, leads to an expansion of seam cell number and co-overexpression of bro-1 and rnt-1 results in massive seam cell hyperplasia. Finally, we find that BRO-1 appears to act independently of RNT-1 in certain situations. These studies provide new insights into the function and regulation of this important cancer-associated DNA-binding complex in stem cells and support the view that Runx/CBFbeta factors have oncogenic potential.
|MeSH||Amino Acid Sequence Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Lineage Cell Proliferation DNA / metabolism Female Gene Expression Regulation, Developmental Humans Male Molecular Sequence Data Mutation / genetics Phenotype Protein Binding Repressor Proteins / genetics Repressor Proteins / metabolism* Sequence Alignment Sequence Homology, Amino Acid Stem Cells / cytology* Stem Cells / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* Up-Regulation|
|WOS Category||DEVELOPMENTAL BIOLOGY|
|C.elegans||tm1183 tm1229 tm658 tm388|