論文 - 詳細
| RRC ID | 36008 |
|---|---|
| 著者 | Terawaki S, Camosseto V, Prete F, Wenger T, Papadopoulos A, Rondeau C, Combes A, Rodriguez Rodrigues C, Vu Manh TP, Fallet M, English L, Santamaria R, Soares AR, Weil T, Hammad H, Desjardins M, Gorvel JP, Santos MA, Gatti E, Pierre P. |
| タイトル | RUN and FYVE domain-containing protein 4 enhances autophagy and lysosome tethering in response to Interleukin-4. |
| ジャーナル | J Cell Biol |
| Abstract |
Autophagy is a key degradative pathway coordinated by external cues, including starvation, oxidative stress, or pathogen detection. Rare are the molecules known to contribute mechanistically to the regulation of autophagy and expressed specifically in particular environmental contexts or in distinct cell types. Here, we unravel the role of RUN and FYVE domain-containing protein 4 (RUFY4) as a positive molecular regulator of macroautophagy in primary dendritic cells (DCs). We show that exposure to interleukin-4 (IL-4) during DC differentiation enhances autophagy flux through mTORC1 regulation and RUFY4 induction, which in turn actively promote LC3 degradation, Syntaxin 17-positive autophagosome formation, and lysosome tethering. Enhanced autophagy boosts endogenous antigen presentation by MHC II and allows host control of Brucella abortus replication in IL-4-treated DCs and in RUFY4-expressing cells. RUFY4 is therefore the first molecule characterized to date that promotes autophagy and influences endosome dynamics in a subset of immune cells. |
| 巻・号 | 210(7) |
| ページ | 1133-52 |
| 公開日 | 2015-9-28 |
| DOI | 10.1083/jcb.201501059 |
| PII | jcb.201501059 |
| PMID | 26416964 |
| PMC | PMC4586740 |
| MeSH | Animals Autophagy / genetics Autophagy / immunology* Brucella abortus / immunology Dendritic Cells / cytology Dendritic Cells / immunology* Interleukin-4 / genetics Interleukin-4 / immunology* Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / immunology* Lysosomes / genetics Lysosomes / immunology* Mechanistic Target of Rapamycin Complex 1 Mice Mice, Knockout Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / immunology Multiprotein Complexes / genetics Multiprotein Complexes / immunology Qa-SNARE Proteins / genetics Qa-SNARE Proteins / immunology TOR Serine-Threonine Kinases / genetics TOR Serine-Threonine Kinases / immunology |
| IF | 8.811 |
| 引用数 | 29 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |