RRC ID 36019
Author Sugiyama T, Thillainadesan G, Chalamcharla VR, Meng Z, Balachandran V, Dhakshnamoorthy J, Zhou M, Grewal SIS.
Title Enhancer of Rudimentary Cooperates with Conserved RNA-Processing Factors to Promote Meiotic mRNA Decay and Facultative Heterochromatin Assembly.
Journal Mol Cell
Abstract Erh1, the fission yeast homolog of Enhancer of rudimentary, is implicated in meiotic mRNA elimination during vegetative growth, but its function is poorly understood. We show that Erh1 and the RNA-binding protein Mmi1 form a stoichiometric complex, called the Erh1-Mmi1 complex (EMC), to promote meiotic mRNA decay and facultative heterochromatin assembly. To perform these functions, EMC associates with two distinct complexes, Mtl1-Red1 core (MTREC) and CCR4-NOT. Whereas MTREC facilitates assembly of heterochromatin islands coating meiotic genes silenced by the nuclear exosome, CCR4-NOT promotes RNAi-dependent heterochromatin domain (HOOD) formation at EMC-target loci. CCR4-NOT also assembles HOODs at retrotransposons and regulated genes containing cryptic introns. We find that CCR4-NOT facilitates HOOD assembly through its association with the conserved Pir2/ARS2 protein, and also maintains rDNA integrity and silencing by promoting heterochromatin formation. Our results reveal connections among Erh1, CCR4-NOT, Pir2/ARS2, and RNAi, which target heterochromatin to regulate gene expression and protect genome integrity.
Volume 61(5)
Pages 747-759
Published 2016-3-3
DOI 10.1016/j.molcel.2016.01.029
PII S1097-2765(16)00082-4
PMID 26942678
PMC PMC4791967
MeSH Carrier Proteins / genetics Carrier Proteins / metabolism* Chromatin Assembly and Disassembly* DNA, Ribosomal / genetics DNA, Ribosomal / metabolism Gene Expression Regulation, Fungal Heterochromatin / genetics Heterochromatin / metabolism* Meiosis* Mutation Nucleic Acid Conformation Protein Binding RNA Interference* RNA Stability* RNA, Fungal / genetics RNA, Fungal / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Retroelements Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism mRNA Cleavage and Polyadenylation Factors / genetics mRNA Cleavage and Polyadenylation Factors / metabolism*
IF 15.584
Times Cited 31
Yeast FY12806