RRC ID 3624
Author Penkner A, Tang L, Novatchkova M, Ladurner M, Fridkin A, Gruenbaum Y, Schweizer D, Loidl J, Jantsch V.
Title The nuclear envelope protein Matefin/SUN-1 is required for homologous pairing in C. elegans meiosis.
Journal Dev. Cell
Abstract We identify a highly specific mutation (jf18) in the Caenorhabditis elegans nuclear envelope protein matefin MTF-1/SUN-1 that provides direct evidence for active involvement of the nuclear envelope in homologous chromosome pairing in C. elegans meiosis. The reorganization of chromatin in early meiosis is disrupted in mtf-1/sun-1(jf18) gonads, concomitant with the absence of presynaptic homolog alignment. Synapsis is established precociously and nonhomologously. Wild-type leptotene/zygotene nuclei show patch-like aggregations of the ZYG-12 protein, which fail to develop in mtf-1/sun-1(jf18) mutants. These patches remarkably colocalize with a component of the cis-acting chromosomal pairing center (HIM-8) rather than the centrosome. Our data on this mtf-1/sun-1 allele challenge the previously postulated role of the centrosome/spindle organizing center in chromosome pairing, and clearly support a role for MTF-1/SUN-1 in meiotic chromosome reorganization and in homolog recognition, possibly by mediating local aggregation of the ZYG-12 protein in meiotic nuclei.
Volume 12(6)
Pages 873-85
Published 2007-6
DOI 10.1016/j.devcel.2007.05.004
PII S1534-5807(07)00199-2
PMID 17543861
MeSH Animals Animals, Genetically Modified Apoptosis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Nucleus / metabolism Chromosome Pairing* DNA Replication Gonads / metabolism In Situ Hybridization, Fluorescence Meiosis* Nuclear Envelope / metabolism* Protein Transport Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / physiology* Recombination, Genetic*
IF 9.616
Times Cited 102
WOS Category DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
C.elegans tm1458