Reference - Detail
| RRC ID | 3627 |
|---|---|
| Author | Sapir A, Choi J, Leikina E, Avinoam O, Valansi C, Chernomordik LV, Newman AP, Podbilewicz B. |
| Title | AFF-1, a FOS-1-regulated fusogen, mediates fusion of the anchor cell in C. elegans. |
| Journal | Dev Cell |
| Abstract |
Cell fusion is fundamental for reproduction and organ formation. Fusion between most C. elegans epithelial cells is mediated by the EFF-1 fusogen. However, fusion between the anchor cell and the utse syncytium that establishes a continuous uterine-vulval tube proceeds normally in eff-1 mutants. By isolating mutants where the anchor-cell fails to fuse, we identified aff-1. AFF-1 ectopic expression results in fusion of cells that normally do not fuse in C. elegans. The fusogen activity of AFF-1 was further confirmed by its ability to fuse heterologous cells. AFF-1 and EFF-1 differ in their fusogenic activity and expression patterns but share eight conserved predicted disulfide bonds in their ectodomains, including a putative TGF-beta-type-I-Receptor domain. We found that FOS-1, the Fos transcription factor ortholog that controls anchor-cell invasion during nematode development, is a specific activator of aff-1-mediated anchor-cell fusion. Thus, FOS-1 links cell invasion and fusion in a developmental cascade. |
| Volume | 12(5) |
| Pages | 683-98 |
| Published | 2007-5-1 |
| DOI | 10.1016/j.devcel.2007.03.003 |
| PII | S1534-5807(07)00103-7 |
| PMID | 17488621 |
| PMC | PMC1975806 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Cell Fusion Cytoplasm / metabolism Embryo, Nonmammalian / cytology Epithelial Cells / cytology Female Insecta / cytology Models, Biological Molecular Sequence Data Mutation / genetics Phenotype Proto-Oncogene Proteins c-fos / chemistry Proto-Oncogene Proteins c-fos / metabolism* Transcription Factors / chemistry Transcription Factors / metabolism* Vulva / cytology Vulva / growth & development |
| IF | 10.092 |
| Times Cited | 71 |
| WOS Category | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2214 |