論文 - 詳細
| RRC ID | 3642 |
|---|---|
| 著者 | Wang X, Wang J, Gengyo-Ando K, Gu L, Sun CL, Yang C, Shi Y, Kobayashi T, Shi Y, Mitani S, Xie XS, Xue D. |
| タイトル | C. elegans mitochondrial factor WAH-1 promotes phosphatidylserine externalization in apoptotic cells through phospholipid scramblase SCRM-1. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Externalization of phosphatidylserine, which is normally restricted to the inner leaflet of plasma membrane, is a hallmark of mammalian apoptosis. It is not known what activates and mediates the phosphatidylserine externalization process in apoptotic cells. Here, we report the development of an annexin V-based phosphatidylserine labelling method and show that a majority of apoptotic germ cells in Caenorhabditis elegans have surface-exposed phosphatidylserine, indicating that phosphatidylserine externalization is a conserved apoptotic event in worms. Importantly, inactivation of the gene encoding either the C. elegans apoptosis-inducing factor (AIF) homologue (WAH-1), a mitochondrial apoptogenic factor, or the C. elegans phospholipid scramblase 1 (SCRM-1), a plasma membrane protein, reduces phosphatidylserine exposure on the surface of apoptotic germ cells and compromises cell-corpse engulfment. WAH-1 associates with SCRM-1 and activates its phospholipid scrambling activity in vitro. Thus WAH-1, after its release from mitochondria during apoptosis, promotes plasma membrane phosphatidylserine externalization through its downstream effector, SCRM-1. |
| 巻・号 | 9(5) |
| ページ | 541-9 |
| 公開日 | 2007-5-1 |
| DOI | 10.1038/ncb1574 |
| PII | ncb1574 |
| PMID | 17401362 |
| MeSH | Animals Annexin A5 / metabolism Apoptosis* Biological Transport Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caspases / genetics Caspases / metabolism Cell Membrane / metabolism Germ Cells / enzymology Germ Cells / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Mitochondria / metabolism Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism* Models, Molecular Mutation Phosphatidylserines / metabolism* Phospholipid Transfer Proteins / genetics Phospholipid Transfer Proteins / metabolism* RNA Interference Staining and Labeling / methods Time Factors |
| IF | 20.042 |
| 引用数 | 84 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm698 tm469 tm805 tm650 tm631 tm624 |