RRC ID 3663
Author Tanaka N, Takahashi H, Kitano H, Matsuoka M, Akao S, Uchimiya H, Komatsu S.
Title Proteome approach to characterize the methylmalonate-semialdehyde dehydrogenase that is regulated by gibberellin.
Journal J. Proteome Res.
Abstract Proteins regulated by gibberellin (GA) in rice were determined by proteome analysis. Proteins extracted from suspension culture cells of slr1, a constitutive GA response mutant of rice, were separated by two-dimensional polyacrylamide gel electrophoresis, and three proteins were greatly accumulated in the mutant. The most up-regulated protein was methylmalonate-semialdehyde dehydrogenase (MMSDH), and the amount of protein was 7-fold that of wild type. In this study, the function of MMSDH in rice was analyzed. MMSDH gene expression in suspension culture cells, roots, and leaf sheaths ofslr1 was higher than that in its wild-type. MMSDH expression in wild-type roots was increased by exogenous GA(3). Analyzed by in situ hybridization, MMSDH mRNA was expressed in root primordia of slr1, where cells are undergoing growth. MMSDH gene expression in the root zone of tissue differentiation was higher than in the elongation zone or meristem. Transgenic rice expressing antisense MMSDH showed that its seminal roots were thinner than that of control, and that the leaf sheath elongation was slightly inhibited compared to control. Concentrations of TCA cycle metabolites were decreased in the antisense plants as compared with the control plants, suggesting that acetyl-CoA was reduced in the antisense plants. These results suggest that one of the regulations by GA signal transduction including SLR1 is the expression of MMSDH, and that MMSDH may play a role in root development and leaf sheath elongation in rice.
Volume 4(5)
Pages 1575-82
Published 2005-9
DOI 10.1021/pr050114f
PMID 16212409
MeSH Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation* Gene Expression Regulation, Enzymologic* Gene Expression Regulation, Plant* Genetic Vectors Gibberellins / chemistry* Gibberellins / metabolism In Situ Hybridization Methylmalonate-Semialdehyde Dehydrogenase (Acylating) / chemistry* Models, Genetic Mutation Oligonucleotides, Antisense / chemistry Oryza / enzymology* Oryza / genetics* Peptide Mapping Phenotype Plant Leaves / metabolism Plants / metabolism Plants, Genetically Modified Proteins / chemistry Proteome Proteomics / methods* RNA / chemistry RNA, Messenger / metabolism RNA, Ribosomal / chemistry Signal Transduction Time Factors Transgenes Up-Regulation
IF 3.95
Times Cited 16
WOS Category BIOCHEMICAL RESEARCH METHODS
Resource
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