Reference - Detail
|Author||Mori J, Kawabata A, Tang H, Tadagaki K, Mizuguchi H, Kuroda K, Mori Y.|
|Title||Human Herpesvirus-6 U14 Induces Cell-Cycle Arrest in G2/M Phase by Associating with a Cellular Protein, EDD.|
The human herpesvirus-6 (HHV-6) infection induces cell-cycle arrest. In this study, we found that the HHV-6-encoded U14 protein induced cell-cycle arrest at G2/M phase via an association with the cellular protein EDD, a mediator of DNA-damage signal transduction. In the early phase of HHV-6 infection, U14 colocalized with EDD dots in the nucleus, and similar colocalization was also observed in cells transfected with a U14 expression vector. When the carboxyl-terminal region of U14 was deleted, no association of U14 and EDD was observed, and the percentage of cells in G2/M decreased relative to that in cells expressing wild-type U14, indicating that the C-terminal region of U14 and the U14-EDD association are critical for the cell-cycle arrest induced by U14. These results indicate that U14 is a G2/M checkpoint regulator encoded by HHV-6.
|MeSH||Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism Cell Division Cell Line, Tumor Cell Nucleus / pathology Cell Nucleus / virology Checkpoint Kinase 2 / genetics Checkpoint Kinase 2 / metabolism DNA Damage G2 Phase Cell Cycle Checkpoints / genetics* Gene Expression Regulation HEK293 Cells HeLa Cells Herpesvirus 6, Human / genetics* Herpesvirus 6, Human / metabolism Host-Pathogen Interactions Humans Leukocytes, Mononuclear / pathology Leukocytes, Mononuclear / virology* Protein Binding Signal Transduction T-Lymphocytes Ubiquitin-Protein Ligases / genetics* Ubiquitin-Protein Ligases / metabolism Viral Proteins / genetics* Viral Proteins / metabolism Virus Replication / physiology*|
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