RRC ID 3690
Author Hauf S, Biswas A, Langegger M, Kawashima SA, Tsukahara T, Watanabe Y.
Title Aurora controls sister kinetochore mono-orientation and homolog bi-orientation in meiosis-I.
Journal EMBO J.
Abstract Aurora-B kinases are important regulators of mitotic chromosome segregation, where they are required for the faithful bi-orientation of sister chromatids. In contrast to mitosis, sister chromatids have to be oriented toward the same spindle pole in meiosis-I, while homologous chromosomes are bi-oriented. We find that the fission yeast Aurora kinase Ark1 is required for the faithful bi-orientation of sister chromatids in mitosis and of homologous chromosomes in meiosis-I. Unexpectedly, Ark1 is also necessary for the faithful mono-orientation of sister chromatids in meiosis-I, even though the canonical mono-orientation pathway, which depends on Moa1 and Rec8, seems intact. Our data suggest that Ark1 prevents unified sister kinetochores during metaphase-I from merotelic attachment to both spindle poles and thus from being torn apart during anaphase-I, revealing a novel mechanism promoting monopolar attachment. Furthermore, our results provide an explanation for the previously enigmatic observation that fission yeast Shugoshin Sgo2, which assists in loading Aurora to centromeres, and its regulator Bub1 are required for the mono-orientation of sister chromatids in meiosis-I.
Volume 26(21)
Pages 4475-86
Published 2007-10-31
DOI 10.1038/sj.emboj.7601880
PII 7601880
PMID 17932486
PMC PMC2034495
MeSH Alleles Aurora Kinases Cell Nucleus / metabolism Chromatids / ultrastructure Chromosomal Proteins, Non-Histone / physiology Image Processing, Computer-Assisted Kinetochores / metabolism* Meiosis* Mitosis Models, Biological Models, Genetic Phosphoproteins / metabolism Protein-Serine-Threonine Kinases / physiology* Schizosaccharomyces / genetics* Schizosaccharomyces / physiology Schizosaccharomyces pombe Proteins / metabolism Schizosaccharomyces pombe Proteins / physiology Sister Chromatid Exchange
IF 10.557
Times Cited 58
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Yeast FY13765(PZ164) FY13770(PZ170) FY13783(PZ856) FY13785(PZ876) FY13812(PX342) FY13782(PZ809)