RRC ID 3726
Author Nakamura T, Asakawa H, Nakase Y, Kashiwazaki J, Hiraoka Y, Shimoda C.
Title Live observation of forespore membrane formation in fission yeast.
Journal Mol. Biol. Cell
Abstract Sporulation in the fission yeast Schizosaccharomyces pombe is a unique biological process in that the plasma membrane of daughter cells is assembled de novo within the mother cell cytoplasm. A double unit membrane called the forespore membrane (FSM) is constructed dynamically during meiosis. To obtain a dynamic view of FSM formation, we visualized FSM in living cells by using green fluorescent protein fused with Psy1, an FSM-resident protein, together with the nucleus or microtubules. The assembly of FSM initiates in prophase II, and four FSMs in a cell expand in a synchronous manner at the same rate throughout meiosis II. After the meiosis II completes, FSMs continue to expand until closure to form the prespore, a spore precursor. Prespores are initially ellipsoidal, and eventually become spheres. FSM formation was also observed in the sporulation-deficient mutants spo3, spo14, and spo15. In the spo15 mutant, the initiation of FSM formation was completely blocked. In the spo3 mutant, the FSM expanded normally during early meiosis II, but it was severely inhibited during late and postmeiosis, whereas in the spo14 mutant, membrane expansion was more severely inhibited throughout meiosis II. These observations suggest that FSM expansion is composed of two steps, early meiotic FSM expansion and late and post meiotic FSM expansion. Possible regulatory mechanisms of FSM formation in fission yeast are discussed.
Volume 19(8)
Pages 3544-53
Published 2008-8
DOI 10.1091/mbc.e08-04-0414
PII E08-04-0414
PMID 18550796
PMC PMC2488312
MeSH Cell Division Cell Membrane / metabolism* Cell Nucleus / metabolism Gene Expression Regulation, Fungal* Meiosis* Membrane Proteins / metabolism Microscopy / instrumentation Microscopy / methods* Microtubule-Associated Proteins / metabolism Microtubules / metabolism Models, Biological Mutation* Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / metabolism Spindle Apparatus / genetics Spores, Fungal / physiology*
IF 3.905
Times Cited 29
Yeast FY12296(YN68) FY12332(YN104) FY13150(TN106) FY7331(TN200) FY7259(MKL216)