論文 - 詳細
| RRC ID | 37490 |
|---|---|
| 著者 | Sonoki H, Sato T, Endo S, Matsunaga T, Yamaguchi M, Yamazaki Y, Sugatani J, Ikari A. |
| タイトル | Quercetin Decreases Claudin-2 Expression Mediated by Up-Regulation of microRNA miR-16 in Lung Adenocarcinoma A549 Cells. |
| ジャーナル | Nutrients |
| Abstract |
Claudin-2 is highly expressed in human lung adenocarcinoma tissues and cells. Knockdown of claudin-2 decreases cell proliferation and migration. Claudin-2 may be a novel target for lung adenocarcinoma. However, there are no physiologically active substances of foods which decrease claudin-2 expression. We here found that quercetin, a flavonoid present in fruits and vegetables, time- and concentration-dependently decreases claudin-2 expression in lung adenocarcinoma A549 cells. In the present study, we examined what regulatory mechanism is involved in the decrease in claudin-2 expression by quercetin. Claudin-2 expression was decreased by LY-294002, a phosphatidylinositol 3-kinase (PI3-K) inhibitor, and U0126, a MEK inhibitor. These drugs inhibited the phosphorylation of Akt and ERK1/2, which are downstream targets of PI3-K and MEK, respectively. In contrast, quercetin did not inhibit the phosphorylation. Both LY-294002 and U0126 inhibited promoter activity of claudin-2, but quercetin did not. The stability of claudin-2 mRNA was decreased by quercetin. Quercetin increased the expression of microRNA miR-16. An inhibitor of miR-16 rescued quercetin-induced decrease in the claudin-2 expression. These results suggest that quercetin decreases claudin-2 expression mediated by up-regulation of miR-16 expression and instability of claudin-2 mRNA in lung adenocarcinoma cells. |
| 巻・号 | 7(6) |
| ページ | 4578-92 |
| 公開日 | 2015-6-8 |
| DOI | 10.3390/nu7064578 |
| PII | nu7064578 |
| PMID | 26061016 |
| PMC | PMC4488803 |
| MeSH | Adenocarcinoma Adenocarcinoma of Lung Butadienes / pharmacology Cell Line, Tumor Cell Proliferation Chromones / pharmacology Claudins / genetics Claudins / metabolism* Fruit Gene Expression Regulation, Neoplastic* Humans Lung Neoplasms MicroRNAs / genetics MicroRNAs / metabolism* Morpholines / pharmacology Nitriles / pharmacology Phosphatidylinositol 3-Kinases / metabolism Phosphoinositide-3 Kinase Inhibitors Phosphorylation Promoter Regions, Genetic Quercetin / pharmacology* RNA, Messenger / genetics RNA, Messenger / metabolism Up-Regulation Vegetables |
| IF | 4.546 |
| 引用数 | 36 |
| WOS 分野 | NUTRITION & DIETETICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549 |