Reference - Detail
| RRC ID | 37855 |
|---|---|
| Author | Shou ZF, Zhou Q, Cai JR, Chen JH, Yamada K, Miyamoto K. |
| Title | PI-3 kinase pathway can mediate the effect of TGF-beta1 in inducing the expression of SHARP-2 in LLC-PK1 cells. |
| Journal | J Zhejiang Univ Sci B |
| Abstract |
We aim to investigate the effect of transforming growth factor (TGF)-beta1 on the expression of enhancer of split- and hairy-related protein-2 (SHARP-2) messenger RNA (mRNA) and its signaling pathway. In this study, several cell lines including LLC-PK1 (a porcine kidney tubular epithelial cell line), MDCK (Madin-Darby canine kidney) and CTLL-2 (cytotoxic T-lymphocyte line) were treated with recombinant human TGF-beta1, and a series of experiments were carried out, involving Northern blot analysis of total RNA from these cells. Further, several specific chemical inhibitors were applied before TGF-beta1 treatment to probe the signaling pathway. The results showed that TGF-beta1 can significantly up-regulate SHARP-2 mRNA expression in the LLC-PK1 cell line. The peak level of induction was found 2 h after TGF-beta1 stimulation. While one phosphoinositide 3-kinases (PI-3) kinase inhibitor, LY294002, completely blocked the effect of TGF-beta1 on SHARP-2 mRNA expression in LLC-PK1 cells at a low concentration, other inhibitors, including PD98059, staurosporine, AG490, wortmannin, okadaic acid and rapamycin, had no effect. The effect of LY294002 was dose-dependent. We conclude that, in LLC-PK1 cells at least, TGF-beta1 can effectively induce the SHARP-2 mRNA expression and that the PI-3 kinase pathway can mediate this effect. |
| Volume | 10(9) |
| Pages | 702-6 |
| Published | 2009-9-1 |
| DOI | 10.1631/jzus.B0920066 |
| PMID | 19735104 |
| PMC | PMC2738841 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / metabolism* Cell Line Dose-Response Relationship, Drug Epithelial Cells / drug effects Epithelial Cells / metabolism* Kidney Tubules / cytology Kidney Tubules / drug effects Kidney Tubules / metabolism* Mice Phosphatidylinositol 3-Kinases / metabolism* Repressor Proteins / metabolism* Signal Transduction / drug effects Signal Transduction / physiology* Swine Transforming Growth Factor beta1 / administration & dosage* Up-Regulation / drug effects Up-Regulation / physiology |
| IF | 2.082 |
| Times Cited | 0 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MEDICINE, RESEARCH & EXPERIMENTAL |
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | |