論文 - 詳細
| RRC ID | 3792 |
|---|---|
| 著者 | Nukazuka A, Fujisawa H, Inada T, Oda Y, Takagi S. |
| タイトル | Semaphorin controls epidermal morphogenesis by stimulating mRNA translation via eIF2alpha in Caenorhabditis elegans. |
| ジャーナル | Genes Dev |
| Abstract |
Conserved semaphorin-plexin signaling systems govern various aspects of animal development, including axonal guidance in vertebrates and epidermal morphogenesis in Caenorhabditis elegans. Here we provide in vivo evidence that stimulation of mRNA translation via eukaryotic initiation factor 2alpha (eIF2alpha) is an essential downstream event of semaphorin signaling in C. elegans. In semaphorin/plexin mutants, a marked elevation in the phosphorylation of eIF2alpha is observed, which causes translation repression and is causally related to the morphological epidermal phenotype in the mutants. Conversely, removal of constraints on translation by genetically reducing the eIF2alpha phosphorylation largely bypasses requirement for the semaphorin signal in epidermal morphogenesis. We also identify an actin-depolymerizing factor/cofilin, whose expression in the mutants is predominantly repressed, as a major translational target of semaphorin signaling. Thus, our results reveal a physiological significance for translation of mRNAs for cytoskeletal regulators, linking environmental cues to cytoskeletal rearrangement during cellular morphogenesis in vivo. |
| 巻・号 | 22(8) |
| ページ | 1025-36 |
| 公開日 | 2008-4-15 |
| DOI | 10.1101/gad.1644008 |
| PII | 22/8/1025 |
| PMID | 18413715 |
| PMC | PMC2335324 |
| MeSH | 3' Untranslated Regions / metabolism Actin Depolymerizing Factors / genetics Actin Depolymerizing Factors / metabolism Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Down-Regulation Epidermis / growth & development* Eukaryotic Initiation Factor-2 / metabolism* Male Microfilament Proteins / genetics Microfilament Proteins / metabolism Microscopy, Confocal Morphogenesis / genetics* Phosphorylation Protein Biosynthesis* Semaphorins / metabolism* eIF-2 Kinase / metabolism |
| IF | 9.527 |
| 引用数 | 27 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1267 tm629 |