論文 - 詳細
| RRC ID | 38522 |
|---|---|
| 著者 | Thang ND, Yajima I, Kumasaka MY, Ohnuma S, Yanagishita T, Hayashi R, Shekhar HU, Watanabe D, Kato M. |
| タイトル | Barium promotes anchorage-independent growth and invasion of human HaCaT keratinocytes via activation of c-SRC kinase. |
| ジャーナル | PLoS One |
| Abstract |
Explosive increases in skin cancers have been reported in more than 36 million patients with arsenicosis caused by drinking arsenic-polluted well water. This study and previous studies showed high levels of barium as well as arsenic in the well water. However, there have been no reports showing a correlation between barium and cancer. In this study, we examined whether barium (BaCl(2)) may independently have cancer-related effects on human precancerous keratinocytes (HaCaT). Barium (5-50 µM) biologically promoted anchorage-independent growth and invasion of HaCaT cells in vitro. Barium (5 µM) biochemically enhanced activities of c-SRC, FAK, ERK and MT1-MMP molecules, which regulate anchorage-independent growth and/or invasion. A SRC kinase specific inhibitor, protein phosphatase 2 (PP2), blocked barium-mediated promotion of anchorage-independent growth and invasion with decreased c-SRC kinase activity. Barium (2.5-5 µM) also promoted anchorage-independent growth and invasion of fibroblasts (NIH3T3) and immortalized nontumorigenic melanocytes (melan-a), but not transformed cutaneous squamous cell carcinoma (HSC5 and A431) and malignant melanoma (Mel-ret) cells, with activation of c-SRC kinase. Taken together, our biological and biochemical findings newly suggest that the levels of barium shown in drinking well water independently has the cancer-promoting effects on precancerous keratinocytes, fibroblast and melanocytes in vitro. |
| 巻・号 | 6(10) |
| ページ | e25636 |
| 公開日 | 2011-1-1 |
| DOI | 10.1371/journal.pone.0025636 |
| PII | PONE-D-11-06873 |
| PMID | 22022425 |
| PMC | PMC3192110 |
| MeSH | Animals Arsenic / analysis Arsenic / toxicity Bangladesh Barium / analysis Barium / toxicity* CSK Tyrosine-Protein Kinase Cell Adhesion / drug effects Cell Movement / drug effects* Cell Proliferation / drug effects Cell Transformation, Neoplastic / pathology Enzyme Activation / drug effects Extracellular Signal-Regulated MAP Kinases / metabolism Fibroblasts / drug effects Fibroblasts / enzymology Fibroblasts / pathology Focal Adhesion Protein-Tyrosine Kinases / metabolism Humans Keratinocytes / drug effects Keratinocytes / enzymology* Keratinocytes / pathology* Matrix Metalloproteinase 14 / metabolism Melanocytes / drug effects Melanocytes / enzymology Melanocytes / pathology Mice NIH 3T3 Cells Protein Kinase Inhibitors / pharmacology Protein-Tyrosine Kinases / antagonists & inhibitors Protein-Tyrosine Kinases / metabolism* Vietnam Water Pollution / analysis Water Wells / chemistry src-Family Kinases |
| IF | 2.74 |
| 引用数 | 17 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A431 NIH3T3 |