論文 - 詳細
| RRC ID | 38804 |
|---|---|
| 著者 | Ohata H, Seito N, Yoshida K, Momose K. |
| タイトル | Lysophosphatidic acid sensitizes mechanical stress-induced Ca2+ mobilization in cultured human lung epithelial cells. |
| ジャーナル | Life Sci |
| Abstract |
We conformed that lysophosphatidic acid (LPA), which is known to be released from activated platelets, sensitizes response in cytosolic free Ca2+ concentration ([Ca2+]i) to mechanical stimulation in cultured epithelial cells (REPF-LC-AI cells) from human lung carcinoma. [Ca2+]i was transiently increased by spritzing of bath solution onto cells as mechanical stimulation in the presence of LPA with concentration-dependent manner (10-100 nM). The transient increase induced by the mechanical stimulation in the presence of LPA was inhibited by 10 microM Ga3+ or removing extracellular Ca2+, but not by 10 microM nicaridipine, suggesting that LPA sensitizes mechanical stimulation-induced Ca2+ influx through stretch-activated ion channels. Phosphatidic acid (1 microM), but not lysophosphatidycholine (10 microM), histamine (100 nM), bradykinin (10 nM), nor ionomycin (100 nM), caused the same effect as that of LPA. This effect was observed in confluent cells, but not in subconfluent cells. These results show that LPA sensitizes mechanoreceptor-linked response in human lung epithelial cells, suggesting a possibility that LPA affects lung function, in particular, during pathological state. |
| 巻・号 | 58(1) |
| ページ | 29-36 |
| 公開日 | 1996-1-1 |
| DOI | 10.1016/0024-3205(95)02252-x |
| PII | 002432059502252X |
| PMID | 8628108 |
| MeSH | Calcium / metabolism* Cell Count Cells, Cultured Epithelial Cells Epithelium / drug effects Epithelium / metabolism Humans Lung / cytology Lung / drug effects* Lung / metabolism* Lysophospholipids / pharmacology* Mechanoreceptors / drug effects* Mechanoreceptors / physiology Sensitivity and Specificity Signal Transduction / drug effects* Signal Transduction / physiology Stress, Mechanical* |
| IF | 3.647 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RERF-LC-AI(RCB0444) |