| RRC ID |
39153
|
| Author |
Osaki T, Wang CX, Tachibana T, Azuma M, Kitamura M, Nakanishi T.
|
| Title |
Generation and Characterization of Rat Monoclonal Antibodies Against Epidermal Growth Factor Receptor.
|
| Journal |
Monoclon Antib Immunodiagn Immunother
|
| Abstract |
Overexpression of the epidermal growth factor receptor (EGFR) gene and dysregulation of EGFR signaling are observed in various cancer cells, and EGFR is a validated target for cancer therapy. In the present study, we report on the generation of two rat anti-EGFR antibodies (clones 2C2D3 and 4H7F4) by using the rat lymph node method. Flow cytometric analysis and immunofluorescence showed that both antibodies specifically bound to EGFR on the surface of cancer cells. Competitive analysis demonstrated that the epitope of each antibody had no overlap with that of the therapeutic anti-EGFR antibody cetuximab. These results suggest that 2C2D3 and 4H7F4 are potentially useful in EGFR-targeted cancer therapy.
|
| Volume |
34(6)
|
| Pages |
418-22
|
| Published |
2015-12-1
|
| DOI |
10.1089/mab.2015.0034
|
| PMID |
26683181
|
| PMC |
PMC4685498
|
| MeSH |
Animals
Antibodies, Monoclonal / biosynthesis*
Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / immunology
Antibody Specificity
Cell Fusion
Cell Line, Tumor
Enzyme-Linked Immunosorbent Assay
Epitopes / analysis*
Epitopes / genetics
Epitopes / immunology
ErbB Receptors / analysis*
ErbB Receptors / genetics
ErbB Receptors / immunology
Female
Gene Expression
HEK293 Cells
Humans
Hybridomas / cytology
Hybridomas / immunology*
Lymph Nodes / cytology
Lymph Nodes / immunology
Lymphocytes / cytology
Lymphocytes / immunology
Mice
Multiple Myeloma / immunology
Multiple Myeloma / pathology
NIH 3T3 Cells
Peptides / administration & dosage
Peptides / genetics
Peptides / metabolism
Protein Binding
Protein Structure, Tertiary
Rats
Rats, Inbred WKY
|
| Resource |
| Human and Animal Cells |
A431(RCB0202) |
