論文 - 詳細
| RRC ID | 39501 |
|---|---|
| 著者 | Nakatani T, Yamagata K, Kimura T, Oda M, Nakashima H, Hori M, Sekita Y, Arakawa T, Nakamura T, Nakano T. |
| タイトル | Stella preserves maternal chromosome integrity by inhibiting 5hmC-induced γH2AX accumulation. |
| ジャーナル | EMBO Rep |
| Abstract |
In the mouse zygote, Stella/PGC7 protects 5-methylcytosine (5mC) of the maternal genome from Tet3-mediated oxidation to 5-hydroxymethylcytosine (5hmC). Although ablation of Stella causes early embryonic lethality, the underlying molecular mechanisms remain unknown. In this study, we report impaired DNA replication and abnormal chromosome segregation (ACS) of maternal chromosomes in Stella-null embryos. In addition, phosphorylation of H2AX (γH2AX), which has been reported to inhibit DNA replication, accumulates in the maternal chromatin of Stella-null zygotes in a Tet3-dependent manner. Cell culture assays verified that ectopic appearance of 5hmC induces abnormal accumulation of γH2AX and subsequent growth retardation. Thus, Stella protects maternal chromosomes from aberrant epigenetic modifications to ensure early embryogenesis. |
| 巻・号 | 16(5) |
| ページ | 582-9 |
| 公開日 | 2015-5-1 |
| DOI | 10.15252/embr.201439427 |
| PII | embr.201439427 |
| PMID | 25694116 |
| PMC | PMC4428038 |
| MeSH | 5-Methylcytosine / analogs & derivatives Animals Cell Division Cell Line Cells, Cultured Chromosomal Instability* Chromosomal Proteins, Non-Histone Chromosome Aberrations Chromosome Segregation Cytosine / analogs & derivatives* Cytosine / metabolism DNA Methylation DNA Replication DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Dioxygenases Embryo, Mammalian / cytology Embryo, Mammalian / metabolism Epigenesis, Genetic Female Gene Knockout Techniques Histones / metabolism* Male Mice Protein Transport Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism* Zygote / metabolism |
| IF | 7.497 |
| 引用数 | 22 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-EF-MCS-IRES2-Venus (RDB04384) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393). |