論文 - 詳細
| RRC ID | 39578 |
|---|---|
| 著者 | Mrozowska PS, Fukuda M. |
| タイトル | Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. |
| ジャーナル | J Cell Biol |
| Abstract |
MDCK II cells, a widely used model of polarized epithelia, develop into different structures depending on culture conditions: two-dimensional (2D) monolayers when grown on synthetic supports or three-dimensional (3D) cysts when surrounded by an extracellular matrix. The establishment of epithelial polarity is accompanied by transcytosis of the apical marker podocalyxin from the outer plasma membrane to the newly formed apical domain, but its exact route and regulation remain poorly understood. Here, through comprehensive colocalization and knockdown screenings, we identified the Rab GTPases mediating podocalyxin transcytosis and showed that different sets of Rabs coordinate its transport during cell polarization in 2D and 3D structures. Moreover, we demonstrated that different Rab35 effectors regulate podocalyxin trafficking in 2D and 3D environments; trafficking is mediated by OCRL in 2D monolayers and ACAP2 in 3D cysts. Our results give substantial insight into regulation of the transcytosis of this apical marker and highlight differences between trafficking mechanisms in 2D and 3D cell cultures. |
| 巻・号 | 213(3) |
| ページ | 355-69 |
| 公開日 | 2016-5-9 |
| DOI | 10.1083/jcb.201512024 |
| PII | jcb.201512024 |
| PMID | 27138252 |
| PMC | PMC4862332 |
| MeSH | Animals Cell Culture Techniques Cell Membrane / metabolism Cell Polarity Dogs Epithelial Cells / metabolism Gene Knockdown Techniques Madin Darby Canine Kidney Cells Models, Molecular Protein Transport Sialoglycoproteins / metabolism* Transcytosis rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism rab GTP-Binding Proteins / physiology* |
| IF | 8.811 |
| 引用数 | 34 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pEGFP-C1-mouse Rab35 (RDB14197) pEGFP-C1-mouse Rab35(TS/TA) (RDB14198) pEGFP-C1-mouse Rab35(S5A) (RDB14199). |