論文 - 詳細
RRC ID | 39832 |
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著者 | Cui YD, Inanami O, Yamamori T, Niwa K, Nagahata H, Kuwabara M. |
タイトル | FMLP-induced formation of F-actin in HL60 cells is dependent on PI3-K but not on intracellular Ca2+, PKC, ERK or p38 MAPK. |
ジャーナル | Inflamm Res |
Abstract |
OBJECTIVE AND DESIGN:To further understand the mechanisms of signal transduction pathways for the formation of F-actin (polymerization of actin) and the activation of NADPH oxidase in phagocytic cells, the effects of various inhibitors on them were studied. MATERIALS AND METHODS:Differentiated HL60 cells were studied to examine their N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated formation of F-actin and activation of NADPH oxidase following treatment with various inhibitors. These included a protein kinase C (PKC) inhibitor (GF 109203X), a phosphatidylinositide 3 kinase (PI3-K) inhibitor (wortmannin), an extracellular response kinase (ERK) inhibitor (PD 98059), a p38 mitogen-activated protein kinase (MAPK) inhibitor (SB 203580) and an intracellular Ca2+ -chelator (BAPTA-AM). RESULTS:The treatment with wortmannin suppressed the formation of F-actin, with less suppression of the activation of NADPH oxidase. BAPTA-AM and GF 109203X did not attenuate the formation of F-actin but completely inhibited the activation of NADPH oxidase. PD 98059 and SB 203580 partially inhibited the activation of NADPH oxidase without influence on the formation of F-actin. Furthermore, wortmannin but not BAPTA-AM and GF 109203X inhibited the fMLP-induced activation of Akt, which is known to regulate NADPH oxidase. CONCLUSIONS:These results suggest that the formation of F-actin is dependent on PI3-K and independent of PKC, ERK and p38 MAPK as well as the increase in intracellular Ca2+, whereas the activation of NADPH oxidase is partly dependent on ERK, p38 MAPK, Akt regulated by PI3-K, and strongly dependent on the activation of PKC and the increase in intracellular Ca2+. |
巻・号 | 49(12) |
ページ | 684-91 |
公開日 | 2000-12-1 |
DOI | 10.1007/s000110050647 |
PMID | 11211919 |
MeSH | Actins / metabolism* Blotting, Western Calcium / metabolism* Cell Differentiation / drug effects Chelating Agents / pharmacology Enzyme Activation / drug effects Enzyme Activators / pharmacology Enzyme Inhibitors / pharmacology HL-60 Cells Humans Mitogen-Activated Protein Kinases / antagonists & inhibitors Mitogen-Activated Protein Kinases / metabolism* N-Formylmethionine Leucyl-Phenylalanine / pharmacology* NADPH Oxidases / metabolism Phosphatidylinositol 3-Kinases / metabolism* Protein Kinase C / antagonists & inhibitors Protein Kinase C / metabolism* Reactive Oxygen Species / metabolism p38 Mitogen-Activated Protein Kinases |
IF | 3.174 |
引用数 | 16 |
WOS 分野 | IMMUNOLOGY CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | HL60(RCB0041) |