RRC ID 4125
著者 Nambiar RM, Ignatius MS, Henion PD.
タイトル Zebrafish colgate/hdac1 functions in the non-canonical Wnt pathway during axial extension and in Wnt-independent branchiomotor neuron migration.
ジャーナル Mech Dev
Abstract Vertebrate gastrulation involves the coordinated movements of populations of cells. These movements include cellular rearrangements in which cells polarize along their medio-lateral axes leading to cell intercalations that result in elongation of the body axis. Molecular analysis of this process has implicated the non-canonical Wnt/Frizzled signaling pathway that is similar to the planar cell polarity pathway (PCP) in Drosophila. Here we describe a zebrafish mutant, colgate (col), which displays defects in the extension of the body axis and the migration of branchiomotor neurons. Activation of the non-canonical Wnt/PCP pathway in these mutant embryos by overexpressing DeltaNdishevelled, rho kinase2 and van gogh-like protein 2 (vangl2) rescues the extension defects suggesting that col acts as a positive regulator of the non-canonical Wnt/PCP pathway. Further, we show that col normally regulates the caudal migration of nVII facial hindbrain branchiomotor neurons and that the mutant phenotype can be rescued by misexpression of vangl2 independent of the Wnt/PCP pathway. We cloned the col locus and found that it encodes histone deacetylase1 (hdac1). Our previous results and studies by others have implicated hdac1 in repressing the canonical Wnt pathway. Here, we demonstrate novel roles for zebrafish hdac1 in activating non-canonical Wnt/PCP signaling underlying axial extension and in promoting Wnt-independent caudal migration of a subset of hindbrain branchiomotor neurons.
巻・号 124(9-10)
ページ 682-98
公開日 2007-1-1
DOI 10.1016/j.mod.2007.07.003
PII S0925-4773(07)00110-4
PMID 17716875
PMC PMC2701655
MeSH Animals Axons / enzymology Axons / physiology* Body Patterning / genetics Body Patterning / physiology* Cell Movement / genetics Cell Movement / physiology* Cell Polarity / genetics Cell Polarity / physiology Histone Deacetylase 1 Histone Deacetylases / genetics Histone Deacetylases / physiology* Motor Neurons / physiology* Mutation Rhombencephalon / cytology Rhombencephalon / embryology Rhombencephalon / enzymology Signal Transduction / genetics Signal Transduction / physiology* Wnt Proteins / genetics Wnt Proteins / physiology* Zebrafish / embryology* Zebrafish / genetics Zebrafish Proteins / genetics Zebrafish Proteins / physiology*
IF 2.126
引用数 41
WOS 分野 DEVELOPMENTAL BIOLOGY
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