RRC ID 41411
著者 Maruyama Y, Suzuki Y, Kazusaka A, Fujita S.
タイトル Uptake of the dopaminergic neurotoxin, norsalsolinol, into PC12 cells via dopamine transporter.
ジャーナル Arch Toxicol
Abstract The uptake of norsalsolinol, a neurotoxin candidate causing parkinsonism-like symptoms, was studied in PC12 cells. The compound was actively taken up by the PC12 cells, with a Km value of 176.24 +/-9.1 microM and a maximum velocity of 55.6 +/- 7.0 pmol/min per mg protein; norsalsolinol uptake was dependent on the presence of extracellular Na+. The uptake of norsalsolinol was sensitive to two dopamine transporter inhibitors, GBR-12909 and reserpine, but was less sensitive to desipramine, a noradrenaline transporter inhibitor. Dopamine competitively inhibited norsalsolinol uptake into PC12 cells with a Ki value of 271.2 +/- 61.6 microM. These results suggest that norsalsolinol is taken up into PC12 cells mainly by the dopamine transporter.
巻・号 75(4)
ページ 209-13
公開日 2001-6-1
DOI 10.1007/s002040000202
PMID 11482518
MeSH Adrenergic Uptake Inhibitors / pharmacology Animals Cells, Cultured Chromatography, High Pressure Liquid Desipramine / pharmacology Dopamine / metabolism* Dopamine Plasma Membrane Transport Proteins Dopamine Uptake Inhibitors / pharmacology Dose-Response Relationship, Drug Membrane Glycoproteins* Membrane Transport Proteins / metabolism* Nerve Tissue Proteins* PC12 Cells Piperazines / pharmacology Rats Reserpine / pharmacology Reverse Transcriptase Polymerase Chain Reaction Salsoline Alkaloids / metabolism* Salsoline Alkaloids / toxicity
IF 5.059
引用数 2
WOS 分野 TOXICOLOGY
リソース情報
ヒト・動物細胞 PC-12(RCB0009)