論文 - 詳細
| RRC ID | 41462 |
|---|---|
| 著者 | Onodera S, Nishihira J, Iwabuchi K, Koyama Y, Yoshida K, Tanaka S, Minami A. |
| タイトル | Macrophage migration inhibitory factor up-regulates matrix metalloproteinase-9 and -13 in rat osteoblasts. Relevance to intracellular signaling pathways. |
| ジャーナル | J Biol Chem |
| Abstract |
Neutral matrix metalloproteinases (MMPs) play an important role in bone matrix degradation accompanied by bone remodeling. We herein show for the first time that macrophage migration inhibitory factor (MIF) up-regulates MMP-13 (collagenase-3) mRNA of rat calvaria-derived osteoblasts. The mRNA up-regulation was seen at 3 h in response to MIF (10 microg/ml), reached the maximum level at 6-12 h, and returned to the basal level at 36 h. MMP-13 mRNA up-regulation was preceded by up-regulation of c-jun and c-fos mRNA. Tissue inhibitor of metalloproteinase (TIMP)-1 and MMP-9 (92-kDa type IV collagenase) were also up-regulated, but to a lesser extent. The MMP-13 mRNA up-regulation was significantly suppressed by genistein, herbimycin A and 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine. Similarly, a selective mitogen-activated protein kinase (MAPK) kinase (MEK)1/2 inhibitor (PD98059) and c-jun/activator protein (AP)-1 inhibitor (curcumin) suppressed MMP-13 mRNA up-regulation induced by MIF. The mRNA levels of c-jun and c-fos in response to MIF were also inhibited by PD98059. Consistent with these results, MIF stimulated phosphorylation of tyrosine, autophosphorylation of Src, activation of Ras, activation of extracellular signal-regulated kinases (ERK) 1/2, a MAPK, but not c-Jun N-terminal kinase or p38, and phosphorylation of c-Jun. Osteoblasts obtained from calvariae of newborn JunAA mice, defective in phosphorylation of c-Jun, or newborn c-Fos knockout (Fos -/- ) mice, showed much less induction of MMP-13 with the addition of MIF than osteoblasts obtained from wild-type or littermate control mice. Taken together, these results suggest that MIF increases the MMP-13 mRNA level of rat osteoblasts via the Src-related tyrosine kinase-, Ras-, ERK1/2-, and AP-1-dependent pathway. |
| 巻・号 | 277(10) |
| ページ | 7865-74 |
| 公開日 | 2002-3-8 |
| DOI | 10.1074/jbc.M106020200 |
| PII | S0021-9258(19)36377-X |
| PMID | 11751895 |
| MeSH | Animals Bone and Bones / metabolism Collagenases / biosynthesis* Collagenases / metabolism Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Fibroblasts / enzymology Fibroblasts / metabolism Flavonoids / pharmacology Genes, Dominant MAP Kinase Kinase 1 MAP Kinase Kinase 2 Macrophage Migration-Inhibitory Factors / metabolism* Matrix Metalloproteinase 13 Matrix Metalloproteinase 9 / biosynthesis* Mitogen-Activated Protein Kinase Kinases / metabolism Mitogen-Activated Protein Kinases / metabolism Osteoblasts / enzymology* Osteoblasts / metabolism* Phosphorylation Protein Binding Protein Serine-Threonine Kinases / metabolism Protein-Tyrosine Kinases / metabolism Proto-Oncogene Proteins c-jun / metabolism RNA, Messenger / metabolism Rats Recombinant Proteins / metabolism Signal Transduction* Time Factors Transcription Factor AP-1 / metabolism Tyrosine / metabolism Up-Regulation* p38 Mitogen-Activated Protein Kinases src-Family Kinases / metabolism |
| IF | 4.238 |
| 引用数 | 133 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |