RRC ID 415
Author Huang X, Huang P, Robinson MK, Stern MJ, Jin Y.
Title UNC-71, a disintegrin and metalloprotease (ADAM) protein, regulates motor axon guidance and sex myoblast migration in C. elegans.
Journal Development
Abstract The migration of cells and growth cones is a process that is guided by extracellular cues and requires the controlled remodeling of the extracellular matrix along the migratory path. The ADAM proteins are important regulators of cellular adhesion and recognition because they can combine regulated proteolysis with modulation of cell adhesion. We report that the C. elegans gene unc-71 encodes a unique ADAM with an inactive metalloprotease domain. Loss-of-function mutations in unc-71 cause distinct defects in motor axon guidance and sex myoblast migration. Many unc-71 mutations affect the disintegrin and the cysteine-rich domains, supporting a major function of unc-71 in cell adhesion. UNC-71 appears to be expressed in a selected set of cells. Genetic mosaic analysis and tissue-specific expression studies indicate that unc-71 acts in a cell non-autonomous manner for both motor axon guidance and sex myoblast migration. Finally, double mutant analysis of unc-71 with other axon guidance signaling molecules suggests that UNC-71 probably functions in a combinatorial manner with integrins and UNC-6/netrin to provide distinct axon guidance cues at specific choice points for motoneurons.
Volume 130(14)
Pages 3147-61
Published 2003-7
PMID 12783787
MeSH Alleles Animals Axons Caenorhabditis elegans Caenorhabditis elegans Proteins / chemistry* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Adhesion Cell Movement Cloning, Molecular Cysteine / chemistry Green Fluorescent Proteins Integrins / metabolism Luminescent Proteins / metabolism Membrane Glycoproteins / chemistry* Metalloendopeptidases / chemistry* Metalloendopeptidases / genetics Metalloendopeptidases / physiology* Models, Genetic Motor Neurons / physiology* Mutation Myoblasts / metabolism* Nerve Growth Factors / metabolism Netrin-1 Phenotype Protein Structure, Tertiary Tissue Distribution Transgenes Tumor Suppressor Proteins
IF 5.413
Times Cited 48
C.elegans tm347 tm353