論文 - 詳細
| RRC ID | 41803 |
|---|---|
| 著者 | Ueda K, Ohta Y, Hosoya H. |
| タイトル | The carboxy-terminal pleckstrin homology domain of ROCK interacts with filamin-A. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The small GTPase Rho and its effector ROCK/Rho-kinase regulate actin cytoskeletal reorganization through phosphorylation of the regulatory light chain of myosin II. We previously reported that ROCK co-purified with the actin-binding protein filamin-A from HeLa cells. Here, we show that the pleckstrin homology (PH) domain of ROCK, but not the kinase or coiled-coil domain, interacts with filamin-A. We also determined that the PH domain of ROCK binds to the carboxy-terminal region of filamin-A containing the last 24th repeat. ROCK co-localized with filamin-A at the protrusive cell membranes of HeLa cells. |
| 巻・号 | 301(4) |
| ページ | 886-90 |
| 公開日 | 2003-2-21 |
| DOI | 10.1016/s0006-291x(03)00048-2 |
| PII | S0006291X03000482 |
| PMID | 12589795 |
| MeSH | Binding Sites Blood Proteins / chemistry Blood Proteins / genetics Blood Proteins / metabolism Cell Membrane / metabolism Contractile Proteins / chemistry Contractile Proteins / genetics Contractile Proteins / metabolism* Filamins HeLa Cells Humans Intracellular Signaling Peptides and Proteins Microfilament Proteins / chemistry Microfilament Proteins / genetics Microfilament Proteins / metabolism* Mutation Phosphoproteins / chemistry Phosphoproteins / genetics Phosphoproteins / metabolism Protein Serine-Threonine Kinases / chemistry* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Protein Structure, Tertiary Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Repetitive Sequences, Amino Acid rho-Associated Kinases |
| IF | 2.985 |
| 引用数 | 34 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |