RRC ID 41884
著者 Yamazaki K, Yamada E, Kanaji Y, Yanagisawa T, Kato Y, Sato K, Takano K, Sakasegawa Y, Kaneko K.
タイトル Stimulation of cellular prion protein expression by TSH in human thyrocytes.
ジャーナル Biochem Biophys Res Commun
Abstract The cellular isoform of prion protein (PrP(C)) is a cell-surface glycosyl-phosphatidylinositol-anchored protein which is ubiquitously expressed on the cell membrane. It may function as a cell receptor or as a cell adhesion molecule. Thyroid follicles, obtained from patients with Graves' disease at thyroidectomy, were cultured in F-12/RPMI-1640 medium supplemented with 0.5% fetal bovine serum and bovine thyroid stimulating hormone (bTSH). Northern blot analyses revealed that bTSH increased the steady-state expression levels of PrP mRNA in a time- and dose-dependent manner. This increase was reproduced by dibutyryl-cAMP and 12-decanoylphorbol-13-acetate. The mRNA expression was greater in thyroid follicles in suspension culture than in thyrocytes cultured in a monolayer. These findings suggest that TSH stimulates PrP mRNA expression in thyrocytes through the protein kinase A and C pathways. The greater mRNA expression in thyroid follicles than in monolayer cells suggests that PrP(C) may be involved in structure formation or maintenance of thyroid follicles.
巻・号 305(4)
ページ 1034-9
公開日 2003-6-13
DOI 10.1016/s0006-291x(03)00801-5
PII S0006291X03008015
PMID 12767934
MeSH Animals Cells, Cultured Gene Expression Profiling Gene Expression Regulation Graves Disease / genetics Graves Disease / metabolism Humans Mice Oligonucleotide Array Sequence Analysis PrPC Proteins / biosynthesis* PrPC Proteins / genetics RNA, Messenger / biosynthesis Thyroid Gland / anatomy & histology Thyroid Gland / drug effects Thyroid Gland / metabolism* Thyrotropin / pharmacology* Tumor Cells, Cultured Up-Regulation
IF 2.985
引用数 5
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 NB9(RCB0477) NB69(RCB0480) K562