RRC ID 41945
著者 Fukami T, Fukuhara H, Kuramochi M, Maruyama T, Isogai K, Sakamoto M, Takamoto S, Murakami Y.
タイトル Promoter methylation of the TSLC1 gene in advanced lung tumors and various cancer cell lines.
ジャーナル Int J Cancer
Abstract We previously identified TSLC1, a tumor suppressor gene in human nonsmall cell lung cancer (NSCLC). TSLC1 belongs to immunoglobulin superfamily molecules and is involved in cell adhesion. Loss of TSLC1 expression was strongly correlated with the promoter hypermethylation in several NSCLC cell lines. Here, we examined the methylation status of the TSLC1 gene promoter in 48 primary NSCLC tumors by bisulfite SSCP in combination with bisulfite sequencing. Six CpG sites around the promoter regions were significantly methylated in 21 of 48 primary NSCLC tumors (44%). Promoter methylation was more likely to be observed in relatively advanced tumors with TNM classification of pT2, pT3 or pT4 (19 of 33, 58%) than in those with pT1 (2 of 15, 13%), suggesting that alteration of TSLC1 would be involved in the progression of human NSCLC. Loss of TSLC1 expression was also observed in 20 of 46 (43%) human cancer cell lines, including those from esophageal (3 of 3), gastric (8 of 9), ovarian (2 of 5), endometrial (2 of 2), breast (1 of 3), colorectal (2 of 8) and small cell lung cancers (2 of 10). Combined analysis of promoter methylation and the allelic state in these cell lines indicated that the TSLC1 gene was often silenced not only by mono-allelic methylation associated with loss of the other allele but also through bi-allelic methylation. These results suggest that alteration of TSLC1 would be involved in advanced NSCLC as well as in many other human cancers.
巻・号 107(1)
ページ 53-9
公開日 2003-10-20
DOI 10.1002/ijc.11348
PMID 12925956
MeSH Adenocarcinoma / genetics Adenocarcinoma / surgery Adult Alleles Brain / metabolism Brain / pathology Carcinoma, Non-Small-Cell Lung / genetics* Carcinoma, Non-Small-Cell Lung / surgery Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / surgery Case-Control Studies Cell Adhesion Molecule-1 Cell Adhesion Molecules CpG Islands DNA Methylation* DNA Primers / chemistry Genes, Tumor Suppressor Humans Immunoglobulins* Loss of Heterozygosity Lung / metabolism Lung / pathology Lung Neoplasms / genetics* Lung Neoplasms / surgery Membrane Proteins* Microsatellite Repeats Neoplasms / genetics Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Promoter Regions, Genetic* Proteins / genetics* Tumor Cells, Cultured Tumor Suppressor Proteins
IF 5.145
引用数 82
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 MKN7(RCB0999) GCIY(RCB0555) OCUB-M(RCB0881) OCUB-F(RCB0882) JHOC-5(RCB1520) JHOS-2(RCB1521) JHUEM-2(RCB1551) JHUAS-1(RCB1544) EC-GI-10(RCB0774) JHUCS-1(RCB1547) CACO-2(RCB0988)