Reference - Detail
|Author||Banfield KL, Gomez TA, Lee W, Clarke S, Larsen PL.|
|Title||Protein-repair and hormone-signaling pathways specify dauer and adult longevity and dauer development in Caenorhabditis elegans.|
|Journal||J. Gerontol. A Biol. Sci. Med. Sci.|
Protein damage that accumulates during aging can be mitigated by a repair methyltransferase, the l-isoaspartyl-O-methyltransferase. In Caenorhabditis elegans, the pcm-1 gene encodes this enzyme. In response to pheromone, we show that pcm-1 mutants form fewer dauer larvae with reduced survival due to loss of the methyltransferase activity. Mutations in daf-2, an insulin/insulin-like growth factor-1-like receptor, and daf-7, a transforming growth factor-beta-like ligand, modulate pcm-1 dauer defects. Additionally, daf-2 and daf-7 mutant dauer larvae live significantly longer than wild type. Although dauer larvae are resistant to many environmental stressors, a proportionately larger decrease in dauer larvae life spans occurred at 25 degrees C compared to 20 degrees C than in adult life span. At 25 degrees C, mutation of the daf-7 or pcm-1 genes does not change adult life span, whereas mutation of the daf-2 gene and overexpression of PCM-1 increases adult life span. Thus, there are both overlapping and distinct mechanisms that specify dauer and adult longevity.
|MeSH||Aging / genetics Aging / metabolism* Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / physiology* Epistasis, Genetic Forkhead Transcription Factors Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology* Genes, Helminth Larva / physiology Longevity / genetics Longevity / physiology Methyltransferases / genetics Methyltransferases / physiology* Mutation Pheromones Protein D-Aspartate-L-Isoaspartate Methyltransferase / genetics Receptor, Insulin / physiology Signal Transduction Transcription Factors / physiology Transforming Growth Factor beta / physiology|
|WOS Category||GERONTOLOGY GERIATRICS & GERONTOLOGY|