論文 - 詳細
| RRC ID | 42116 |
|---|---|
| 著者 | Ishii T, Ohshima S, Ishida T, Mima T, Tabunoki Y, Kobayashi H, Maeda M, Uede T, Liaw L, Kinoshita N, Kawase I, Saeki Y. |
| タイトル | Osteopontin as a positive regulator in the osteoclastogenesis of arthritis. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
We examined the role of osteopontin (OPN) in the osteoclastogenesis of arthritis using collagen-induced arthritis (CIA). Cells from arthritic joints of wild-type (OPN +/+) mice spontaneously developed bone-resorbing osteoclast-like cells (OCLs). The cultured cells showed an enhanced expression of receptor activator of nuclear factor kappaB ligand (RANKL) and a decreased expression of osteoprotegerin (OPG). The addition of OPG reduced the number of OCLs, indicating that the osteoclastogenesis depends on the RANK/RANKL/OPG system. The cells also produced OPN abundantly and anti-OPN neutralizing antibodies suppressed the development of OCLs. Moreover, the addition of OPN increased the expression of RANKL and augmented differentiation of OCLs from OPN-deficient (OPN -/-) cells. OPN, like the combination of 1alpha,25-dihydroxyvitamin D(3) and dexamethasone, also enhanced the RANKL expression and decreased OPG expression in a stromal cell line, ST2. These results suggest that OPN acts as a positive regulator in the osteoclastogenesis of arthritis through the RANK/RANKL/OPG system. |
| 巻・号 | 316(3) |
| ページ | 809-15 |
| 公開日 | 2004-4-9 |
| DOI | 10.1016/j.bbrc.2004.02.124 |
| PII | S0006291X0400395X |
| PMID | 15033472 |
| MeSH | Animals Arthritis / etiology Arthritis / metabolism* Calcitriol / metabolism Carrier Proteins / metabolism Cell Differentiation Cell Line Cells, Cultured Collagen / metabolism Dexamethasone / pharmacology Dose-Response Relationship, Drug Gene Expression Regulation* Glycoproteins / metabolism Membrane Glycoproteins / metabolism Mice Mice, Inbred DBA Mice, Transgenic Models, Genetic Osteoclasts / metabolism Osteopontin Osteoprotegerin RANK Ligand RNA, Messenger / metabolism Receptor Activator of Nuclear Factor-kappa B Receptors, Cytoplasmic and Nuclear / metabolism Receptors, Tumor Necrosis Factor Recombinant Proteins / chemistry Reverse Transcriptase Polymerase Chain Reaction Sialoglycoproteins / physiology* Stromal Cells / cytology |
| IF | 2.985 |
| 引用数 | 38 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |