Reference - Detail
|Author||Ghila L, Gomez M.|
|Title||The evolutionarily conserved gene LNP-1 is required for synaptic vesicle trafficking and synaptic transmission.|
|Journal||Eur. J. Neurosci.|
The control of vesicle-mediated transport in nerve cells is of great importance in the function, development and maintenance of synapse. In this paper, we characterize the new Caenorhabditis elegans gene, lnp-1. The lnp-1 gene is broadly distributed in many neuronal structures and its localization is dependent of the UNC-104/kinesin protein. Deletion mutations in lnp-1 result in increased resistance to aldicarb, an acetylcholinesterase inhibitor, and in locomotor defects. However, sensitivity to levamisole, a nicotinic agonist which, unlike aldicarb, only affects postsynaptic function, was similar to that of wild-type animals, suggesting a presynaptic function for LNP-1 in neurotransmission. The mislocalization of presynaptic proteins, such as synaptobrevin-1 or RAB-3, in lnp-1 mutants further supports this hypothesis. In summary, our studies suggest that LNP-1 plays a role in synaptogenesis by regulating vesicular transport or localization.
|MeSH||Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / isolation & purification Caenorhabditis elegans Proteins / metabolism* Cholinesterase Inhibitors / pharmacology Conserved Sequence Evolution, Molecular Mutation / genetics Nerve Tissue Proteins / genetics Nerve Tissue Proteins / isolation & purification Nerve Tissue Proteins / metabolism* Nervous System / metabolism Nervous System / ultrastructure Nicotinic Agonists / pharmacology Presynaptic Terminals / metabolism* Presynaptic Terminals / ultrastructure Protein Transport / physiology Synaptic Transmission / physiology* Synaptic Vesicles / metabolism* Synaptic Vesicles / ultrastructure Vesicle-Associated Membrane Protein 1 / metabolism rab3 GTP-Binding Proteins / genetics rab3 GTP-Binding Proteins / metabolism|