| RRC ID |
4231
|
| Author |
Goodyer W, Kaitna S, Couteau F, Ward JD, Boulton SJ, Zetka M.
|
| Title |
HTP-3 links DSB formation with homolog pairing and crossing over during C. elegans meiosis.
|
| Journal |
Dev Cell
|
| Abstract |
Repair of the programmed meiotic double-strand breaks (DSBs) that initiate recombination must be coordinated with homolog pairing to generate crossovers capable of directing chromosome segregation. Chromosome pairing and synapsis proceed independently of recombination in worms and flies, suggesting a paradoxical lack of coregulation. Here, we find that the meiotic axis component HTP-3 links DSB formation with homolog pairing and synapsis. HTP-3 forms complexes with the DSB repair components MRE-11/RAD-50 and the meiosis-specific axis component HIM-3. Loss of htp-3 or mre-11 recapitulates meiotic phenotypes consistent with a failure to generate DSBs, suggesting that HTP-3 associates with MRE-11/RAD-50 in a complex required for meiotic DSB formation. Loss of HTP-3 eliminates HIM-3 localization to axes and HIM-3-dependent homolog alignment, synapsis, and crossing over. Our study reveals a mechanism for coupling meiotic DSB formation with homolog pairing through the essential participation of an axis component with complexes mediating both processes.
|
| Volume |
14(2)
|
| Pages |
263-74
|
| Published |
2008-2-1
|
| DOI |
10.1016/j.devcel.2007.11.016
|
| PII |
S1534-5807(07)00438-8
|
| PMID |
18267094
|
| MeSH |
Animals
Caenorhabditis elegans / cytology*
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism*
Cell Cycle Proteins / metabolism*
Chromatin / metabolism
Chromosome Pairing*
Chromosome Positioning
Crossing Over, Genetic*
DNA Breaks, Double-Stranded*
DNA Repair
Meiosis*
Mutation / genetics
Protein Binding
Protein Transport
RNA Interference
|
| IF |
10.092
|
| Times Cited |
109
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm1086 |
