RRC ID 42533
著者 Biswas KK, Tancharoen S, Sarker KP, Kawahara K, Hashiguchi T, Maruyama I.
タイトル Cepharanthine triggers apoptosis in a human hepatocellular carcinoma cell line (HuH-7) through the activation of JNK1/2 and the downregulation of Akt.
ジャーナル FEBS Lett
Abstract Cepharanthine (CEP), a biscoclaurine alkaloid, has been reported to induce cell death, however, the molecular mechanism of this phenomenon remains unclear. We herein report that CEP induced apoptosis in HuH-7 cells through nuclear fragmentation, DNA ladder formation, cytochrome c release, caspase-3 activation and poly-(ADP-ribose)-polymerase cleavage. CEP triggered the generation of reactive oxygen intermediates, the activation of mitogen activated protein kinase (MAPK) p38, JNK1/2 and p44/42, and the downregulation of protein kinase B/Akt. Antioxidants and SP600125, an inhibitor of JNK1/2, but not inhibitors of p38 MAPK and MEK1/2, significantly prevented cell death, thus implying that reactive oxygen species and JNK1/2 play crucial roles in the CEP-induced apoptosis of HuH-7 cells.
巻・号 580(2)
ページ 703-10
公開日 2006-1-23
DOI 10.1016/j.febslet.2005.12.048
PII S0014-5793(05)01531-0
PMID 16412424
MeSH Alkaloids / metabolism* Animals Anthracenes / metabolism Anti-Inflammatory Agents, Non-Steroidal / metabolism* Apoptosis / physiology* Benzylisoquinolines Carcinoma, Hepatocellular* Caspase 3 Caspases / metabolism Cell Line, Tumor Collagen Type XI / metabolism Cytochromes c / metabolism Down-Regulation Enzyme Activation Enzyme Inhibitors / metabolism Humans Liver Neoplasms* Mitogen-Activated Protein Kinase 8 / metabolism* Mitogen-Activated Protein Kinase 9 / metabolism* Proto-Oncogene Proteins c-akt / metabolism* Reactive Oxygen Species / metabolism p38 Mitogen-Activated Protein Kinases / metabolism
IF 3.057
引用数 40
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 HuH-7(RCB1366)