論文 - 詳細
RRC ID | 42533 |
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著者 | Biswas KK, Tancharoen S, Sarker KP, Kawahara K, Hashiguchi T, Maruyama I. |
タイトル | Cepharanthine triggers apoptosis in a human hepatocellular carcinoma cell line (HuH-7) through the activation of JNK1/2 and the downregulation of Akt. |
ジャーナル | FEBS Lett |
Abstract |
Cepharanthine (CEP), a biscoclaurine alkaloid, has been reported to induce cell death, however, the molecular mechanism of this phenomenon remains unclear. We herein report that CEP induced apoptosis in HuH-7 cells through nuclear fragmentation, DNA ladder formation, cytochrome c release, caspase-3 activation and poly-(ADP-ribose)-polymerase cleavage. CEP triggered the generation of reactive oxygen intermediates, the activation of mitogen activated protein kinase (MAPK) p38, JNK1/2 and p44/42, and the downregulation of protein kinase B/Akt. Antioxidants and SP600125, an inhibitor of JNK1/2, but not inhibitors of p38 MAPK and MEK1/2, significantly prevented cell death, thus implying that reactive oxygen species and JNK1/2 play crucial roles in the CEP-induced apoptosis of HuH-7 cells. |
巻・号 | 580(2) |
ページ | 703-10 |
公開日 | 2006-1-23 |
DOI | 10.1016/j.febslet.2005.12.048 |
PII | S0014-5793(05)01531-0 |
PMID | 16412424 |
MeSH | Alkaloids / metabolism* Animals Anthracenes / metabolism Anti-Inflammatory Agents, Non-Steroidal / metabolism* Apoptosis / physiology* Benzylisoquinolines Carcinoma, Hepatocellular* Caspase 3 Caspases / metabolism Cell Line, Tumor Collagen Type XI / metabolism Cytochromes c / metabolism Down-Regulation Enzyme Activation Enzyme Inhibitors / metabolism Humans Liver Neoplasms* Mitogen-Activated Protein Kinase 8 / metabolism* Mitogen-Activated Protein Kinase 9 / metabolism* Proto-Oncogene Proteins c-akt / metabolism* Reactive Oxygen Species / metabolism p38 Mitogen-Activated Protein Kinases / metabolism |
IF | 3.057 |
引用数 | 40 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | HuH-7(RCB1366) |