論文 - 詳細
| RRC ID | 42538 |
|---|---|
| 著者 | Hashimoto O, Ushiro Y, Sekiyama K, Yamaguchi O, Yoshioka K, Mutoh K, Hasegawa Y. |
| タイトル | Impaired growth of pancreatic exocrine cells in transgenic mice expressing human activin betaE subunit. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Activins, TGF-beta superfamily members, have multiple functions in a variety of cells and tissues. Recently, additional activin beta subunit genes, betaC and betaE, have been identified. To explore the role of activin E, we created transgenic mice overexpressing human activin betaE subunit. There were pronounced differences in the pancreata of the transgenic animals as compared with their wild-type counterparts. Pancreatic weight, expressed relative to total body weight, was significantly reduced. Histologically, adipose replacement of acini in the exocrine pancreas was observed. There was a significant decrease in the number of PCNA-positive cells in the acinar cells, indicating reduced proliferation in the exocrine pancreas of the transgenic mice. However, quantitative pancreatic morphometry showed that the total number and mass of the islets of the transgenic mice were comparable with those of the nontransgenic control mice. Our findings suggest a role for activin E in regulating the proliferation of pancreatic exocrine cells. |
| 巻・号 | 341(2) |
| ページ | 416-24 |
| 公開日 | 2006-3-10 |
| DOI | 10.1016/j.bbrc.2005.12.205 |
| PII | S0006-291X(06)00043-X |
| PMID | 16426570 |
| MeSH | Animals Blotting, Northern Blotting, Western Body Weight Cell Line DNA / chemistry DNA Primers / chemistry DNA, Complementary / metabolism Escherichia coli / metabolism Exocrine Glands / metabolism* Female Gene Expression Regulation* Gene Library Glutathione Transferase / metabolism Humans Immunohistochemistry Inhibin-beta Subunits / genetics* Inhibin-beta Subunits / metabolism Ki-67 Antigen / biosynthesis Liver / metabolism Male Mice Mice, Inbred C57BL Mice, Transgenic Organ Size Pancreas / cytology* Phenotype Plasmids / metabolism Polymerase Chain Reaction Recombinant Proteins / chemistry Transfection Transgenes |
| IF | 2.985 |
| 引用数 | 28 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |