論文 - 詳細
| RRC ID | 42564 |
|---|---|
| 著者 | Inoue T, Kon T, Ajima R, Ohkura R, Tani M, Yokota J, Sutoh K. |
| タイトル | MYO18B interacts with the proteasomal subunit Sug1 and is degraded by the ubiquitin-proteasome pathway. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
MYO18B is a class XVIIIB unconventional myosin encoded by a candidate tumor suppressor gene. To gain insights into the cellular function of this protein, we searched for MYO18B-interacting proteins by a yeast two-hybrid screen. Sug1, a 19S regulator subunit of the 26S proteasome, was identified as a binding partner of the C-terminal tail region of MYO18B. The association of MYO18B with Sug1 was further confirmed by GST pull-down, co-immunoprecipitation, and immunocytochemistry. Furthermore, proteasome dysfunction by a proteasome inhibitor or siRNA-mediated knock-down of Sug1 caused the up-regulation of MYO18B protein and MYO18B was polyubiquitinated in vivo. Collectively, these results suggested that MYO18B is a substrate for proteasomal degradation. |
| 巻・号 | 342(3) |
| ページ | 829-34 |
| 公開日 | 2006-4-14 |
| DOI | 10.1016/j.bbrc.2006.02.025 |
| PII | S0006-291X(06)00294-4 |
| PMID | 16499872 |
| MeSH | ATPases Associated with Diverse Cellular Activities Adaptor Proteins, Signal Transducing / metabolism* Animals COS Cells Cells, Cultured Chlorocebus aethiops Cytoplasm HeLa Cells Humans LIM Domain Proteins Myosins / metabolism* Proteasome Endopeptidase Complex / chemistry* Proteasome Endopeptidase Complex / metabolism* Protein Binding Protein Processing, Post-Translational* Protein Transport Transcription Factors / metabolism* Tumor Suppressor Proteins / metabolism* Ubiquitins / metabolism* |
| IF | 2.985 |
| 引用数 | 10 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |