| RRC ID |
42564
|
| 著者 |
Inoue T, Kon T, Ajima R, Ohkura R, Tani M, Yokota J, Sutoh K.
|
| タイトル |
MYO18B interacts with the proteasomal subunit Sug1 and is degraded by the ubiquitin-proteasome pathway.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
MYO18B is a class XVIIIB unconventional myosin encoded by a candidate tumor suppressor gene. To gain insights into the cellular function of this protein, we searched for MYO18B-interacting proteins by a yeast two-hybrid screen. Sug1, a 19S regulator subunit of the 26S proteasome, was identified as a binding partner of the C-terminal tail region of MYO18B. The association of MYO18B with Sug1 was further confirmed by GST pull-down, co-immunoprecipitation, and immunocytochemistry. Furthermore, proteasome dysfunction by a proteasome inhibitor or siRNA-mediated knock-down of Sug1 caused the up-regulation of MYO18B protein and MYO18B was polyubiquitinated in vivo. Collectively, these results suggested that MYO18B is a substrate for proteasomal degradation.
|
| 巻・号 |
342(3)
|
| ページ |
829-34
|
| 公開日 |
2006-4-14
|
| DOI |
10.1016/j.bbrc.2006.02.025
|
| PII |
S0006-291X(06)00294-4
|
| PMID |
16499872
|
| MeSH |
ATPases Associated with Diverse Cellular Activities
Adaptor Proteins, Signal Transducing / metabolism*
Animals
COS Cells
Cells, Cultured
Chlorocebus aethiops
Cytoplasm
HeLa Cells
Humans
LIM Domain Proteins
Myosins / metabolism*
Proteasome Endopeptidase Complex / chemistry*
Proteasome Endopeptidase Complex / metabolism*
Protein Binding
Protein Processing, Post-Translational*
Protein Transport
Transcription Factors / metabolism*
Tumor Suppressor Proteins / metabolism*
Ubiquitins / metabolism*
|
| IF |
2.985
|
| 引用数 |
10
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
