| RRC ID |
4283
|
| 著者 |
Rabbitts BM, Ciotti MK, Miller NE, Kramer M, Lawrenson AL, Levitte S, Kremer S, Kwan E, Weis AM, Hermann GJ.
|
| タイトル |
glo-3, a novel Caenorhabditis elegans gene, is required for lysosome-related organelle biogenesis.
|
| ジャーナル |
Genetics
|
| Abstract |
Gut granules are specialized lysosome-related organelles that act as sites of fat storage in Caenorhabditis elegans intestinal cells. We identified mutations in a gene, glo-3, that functions in the formation of embryonic gut granules. Some glo-3(-) alleles displayed a complete loss of embryonic gut granules, while other glo-3(-) alleles had reduced numbers of gut granules. A subset of glo-3 alleles led to mislocalization of gut granule contents into the intestinal lumen, consistent with a defect in intracellular trafficking. glo-3(-) embryos lacking gut granules developed into adults containing gut granules, indicating that glo-3(+) function may be differentially required during development. We find that glo-3(+) acts in parallel with or downstream of the AP-3 complex and the PGP-2 ABC transporter in gut granule biogenesis. glo-3 encodes a predicted membrane-associated protein that lacks obvious sequence homologs outside of nematodes. glo-3 expression initiates in embryonic intestinal precursors and persists almost exclusively in intestinal cells through adulthood. GLO-3GFP localizes to the gut granule membrane, suggesting it could play a direct role in the trafficking events at the gut granule. smg-1(-) suppression of glo-3(-) nonsense alleles indicates that the C-terminal half of GLO-3, predicted to be present in the cytoplasm, is not necessary for gut granule formation. Our studies identify GLO-3 as a novel player in the formation of lysosome-related organelles.
|
| 巻・号 |
180(2)
|
| ページ |
857-71
|
| 公開日 |
2008-10-1
|
| DOI |
10.1534/genetics.108.093534
|
| PII |
genetics.108.093534
|
| PMID |
18780725
|
| PMC |
PMC2567386
|
| MeSH |
Alleles
Amino Acid Sequence
Animals
Caenorhabditis elegans / embryology
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism
Cytoplasmic Granules / metabolism
Gastrointestinal Tract / cytology
Gastrointestinal Tract / metabolism
Genes, Helminth*
Lysosomes / metabolism*
Molecular Sequence Data
Phenotype
|
| IF |
4.015
|
| 引用数 |
25
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 線虫 |
tm920 |
