論文 - 詳細
| RRC ID | 42916 |
|---|---|
| 著者 | Takaya A, Kamio T, Masuda M, Mochizuki N, Sawa H, Sato M, Nagashima K, Mizutani A, Matsuno A, Kiyokawa E, Matsuda M. |
| タイトル | R-Ras regulates exocytosis by Rgl2/Rlf-mediated activation of RalA on endosomes. |
| ジャーナル | Mol Biol Cell |
| Abstract |
R-Ras is a Ras-family small GTPase that regulates various cellular functions such as apoptosis and cell adhesion. Here, we demonstrate a role of R-Ras in exocytosis. By the use of specific anti-R-Ras antibody, we found that R-Ras was enriched on both early and recycling endosomes in a wide range of cell lines. Using a fluorescence resonance energy transfer-based probe for R-Ras activity, R-Ras activity was found to be higher on endosomes than on the plasma membrane. This high R-Ras activity on the endosomes correlated with the accumulation of an R-Ras effector, the Rgl2/Rlf guanine nucleotide exchange factor for RalA, and also with high RalA activity. The essential role played by R-Ras in inducing high levels of RalA activity on the endosomes was evidenced by the short hairpin RNA (shRNA)-mediated suppression of R-Ras and by the expression of R-Ras GAP. In agreement with the reported role of RalA in exocytosis, the shRNA of either R-Ras or RalA was found to suppress calcium-triggered exocytosis in PC12 pheochromocytoma cells. These data revealed that R-Ras activates RalA on endosomes and that it thereby positively regulates exocytosis. |
| 巻・号 | 18(5) |
| ページ | 1850-60 |
| 公開日 | 2007-5-1 |
| DOI | 10.1091/mbc.e06-08-0765 |
| PII | E06-08-0765 |
| PMID | 17344481 |
| PMC | PMC1855010 |
| MeSH | Animals Base Sequence COS Cells Cell Line Chlorocebus aethiops Dogs Endosomes / metabolism* Exocytosis / physiology* Humans Mice PC12 Cells RNA Interference RNA, Small Interfering / genetics Rats Transcription Factors / genetics Transcription Factors / metabolism* rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism* ral GTP-Binding Proteins / antagonists & inhibitors ral GTP-Binding Proteins / genetics ral GTP-Binding Proteins / metabolism* ras Proteins / antagonists & inhibitors ras Proteins / genetics ras Proteins / metabolism* |
| IF | 3.791 |
| 引用数 | 41 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |