RRC ID 4324
Author Zhou S, Opperman K, Wang X, Chen L.
Title unc-44 Ankyrin and stn-2 gamma-syntrophin regulate sax-7 L1CAM function in maintaining neuronal positioning in Caenorhabditis elegans.
Journal Genetics
Abstract The L1 family of single-pass transmembrane cell adhesion molecules (L1CAMs) is conserved from Caenorhabditis elegans and Drosophila to vertebrates and is required for axon guidance, neurite outgrowth, and maintenance of neuronal positions. The extracellular region of L1CAMs mediates cell adhesion via interactions with diverse cell-surface and extracellular matrix proteins. In contrast, less is known regarding the function of the intracellular domains in the L1CAM cytoplasmic tail. Previously, we identified a role of the C. elegans L1CAM homolog, SAX-7, in maintaining neuronal and axonal positioning. Here, we demonstrate that this function is dependent on three conserved motifs that reside in the SAX-7 cytoplasmic tail: (1) the FERM-binding motif, (2) the ankyrin-binding domain, and (3) the PDZ-binding motif. Furthermore, we provide molecular and genetic evidence that UNC-44 ankyrin and STN-2 gamma-syntrophin bind SAX-7 via the respective ankyrin-binding and PDZ-binding motifs to regulate SAX-7 function in maintaining neuronal positioning.
Volume 180(3)
Pages 1429-43
Published 2008-11
DOI 10.1534/genetics.108.091272
PII genetics.108.091272
PMID 18791240
PMC PMC2581946
MeSH Animals Animals, Genetically Modified Ankyrins / genetics Ankyrins / metabolism* Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cells, Cultured Fluorescent Antibody Technique Humans Kidney / metabolism Muscle Proteins / genetics Muscle Proteins / metabolism* Muscle, Striated / physiology Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Neural Cell Adhesion Molecule L1 / physiology* Neural Cell Adhesion Molecules / genetics* Neural Cell Adhesion Molecules / metabolism Neurons / cytology Neurons / physiology* Phenotype Sarcomeres / physiology Two-Hybrid System Techniques
IF 4.075
Times Cited 15
C.elegans tm1448 tm1869