論文 - 詳細
| RRC ID | 4329 |
|---|---|
| 著者 | Kanamori T, Inoue T, Sakamoto T, Gengyo-Ando K, Tsujimoto M, Mitani S, Sawa H, Aoki J, Arai H. |
| タイトル | Beta-catenin asymmetry is regulated by PLA1 and retrograde traffic in C. elegans stem cell divisions. |
| ジャーナル | EMBO J |
| Abstract |
Asymmetric division is an important property of stem cells. In Caenorhabditis elegans, the Wnt/beta-catenin asymmetry pathway determines the polarity of most asymmetric divisions. The Wnt signalling components such as beta-catenin localize asymmetrically to the cortex of mother cells to produce two distinct daughter cells. However, the molecular mechanism to polarize them remains to be elucidated. Here, we demonstrate that intracellular phospholipase A(1) (PLA(1)), a poorly characterized lipid-metabolizing enzyme, controls the subcellular localizations of beta-catenin in the terminal asymmetric divisions of epithelial stem cells (seam cells). In mutants of ipla-1, a single C. elegans PLA(1) gene, cortical beta-catenin is delocalized and the asymmetry of cell-fate specification is disrupted in the asymmetric divisions. ipla-1 mutant phenotypes are rescued by expression of ipla-1 in seam cells in a catalytic activity-dependent manner. Furthermore, our genetic screen utilizing ipla-1 mutants reveals that reduction of endosome-to-Golgi retrograde transport in seam cells restores normal subcellular localization of beta-catenin to ipla-1 mutants. We propose that membrane trafficking regulated by ipla-1 provides a mechanism to control the cortical asymmetry of beta-catenin. |
| 巻・号 | 27(12) |
| ページ | 1647-57 |
| 公開日 | 2008-6-18 |
| DOI | 10.1038/emboj.2008.102 |
| PII | emboj2008102 |
| PMID | 18497747 |
| PMC | PMC2396877 |
| MeSH | Animals Biological Transport Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Cell Count Cell Division* Cell Lineage Cell Polarity Cytoskeletal Proteins / metabolism Female Genes, Helminth Genes, Suppressor Guanine Nucleotide Exchange Factors Mutant Proteins / isolation & purification Mutant Proteins / metabolism Mutation / genetics Phenotype Phospholipases A1 / metabolism* Spindle Apparatus / metabolism Stem Cells / cytology* Subcellular Fractions / metabolism Vulva / cytology beta Catenin / metabolism* rab GTP-Binding Proteins / metabolism |
| IF | 9.889 |
| 引用数 | 30 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm471 |