| RRC ID |
44180
|
| 著者 |
Okamura H, Yang D, Yoshida K, Haneji T.
|
| タイトル |
Protein phosphatase 2A Cα is involved in osteoclastogenesis by regulating RANKL and OPG expression in osteoblasts.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
We examined whether alteration of PP2A Cα expression in osteoblasts is involved in osteoclast differentiation. Reduction of PP2A Cα in MC3T3-E1 cells (shPP2A) decreased receptor activator of nuclear factor κB ligand (RANKL) expression and increased osteoprotegerin (OPG) expression. The conditioned medium from shPP2A cells failed to induce NFATc1 as well as the expression of osteoclast marker genes cathepsin K and osteoclast-associated receptor (OSCAR) in bone marrow macrophage cells. Treatment of bone marrow macrophage cells with the conditioned medium from shPP2A cells impaired osteoclastogenesis. These results suggest that alteration of PP2A Cα expression in osteoblasts modulates the expressions of RANKL and OPG, which are involved in osteoclastogenesis via the NFATc1 transcription factor.
|
| 巻・号 |
587(1)
|
| ページ |
48-53
|
| 公開日 |
2013-1-4
|
| DOI |
10.1016/j.febslet.2012.10.041
|
| PII |
S0014-5793(12)00833-2
|
| PMID |
23183242
|
| MeSH |
3T3 Cells
Animals
Antigens, Differentiation / genetics
Antigens, Differentiation / metabolism
Bone Resorption / metabolism*
Cell Differentiation*
Cell Line, Transformed
Cells, Cultured
Culture Media, Conditioned / metabolism
Gene Expression Regulation
Gene Silencing
Humans
Macrophages / cytology
Macrophages / metabolism
Mice
Mice, Inbred BALB C
NFATC Transcription Factors / metabolism
Osteoblasts / cytology
Osteoblasts / metabolism*
Osteoclasts / cytology
Osteoclasts / metabolism*
Osteoprotegerin / metabolism*
Protein Phosphatase 2 / antagonists & inhibitors
Protein Phosphatase 2 / genetics
Protein Phosphatase 2 / metabolism*
Protein Subunits / antagonists & inhibitors
Protein Subunits / genetics
Protein Subunits / metabolism
RANK Ligand / metabolism*
Specific Pathogen-Free Organisms
|
| IF |
3.057
|
| 引用数 |
16
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
