Reference - Detail
| RRC ID | 44460 |
|---|---|
| Author | Kawanishi M, Ogo S, Ikemoto M, Totsuka Y, Ishino K, Wakabayashi K, Yagi T. |
| Title | Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells. |
| Journal | J Toxicol Sci |
| Abstract |
We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours' treatment with the particles dose-dependently increased the frequency of micronuclei (MN) in the A549 and CHO AA8 cells up to 5.2% and 5.0% at a dose of 200 µg/ml (34 µg/cm²), respectively. In A549 cells, treatment with the nano-particles (2 µg/ml) for 1 hr induced H2AX phosphorylation, which is suggestive of DNA double strand breaks (DSB). Treating CHO AA8 cells with 2 µg/ml (0.34 µg/cm²) magnetite for 1 hour resulted in a five times higher frequency of sister chromatid exchange (SCE) than the control level. We detected reactive oxygen species (ROS) in CHO cells treated with the particles. These findings indicate that magnetite nano-particles induce ROS in mammalian cells, leading to the direct or indirect induction of DSB, followed by clastogenic events including MN and SCE. |
| Volume | 38(3) |
| Pages | 503-11 |
| Published | 2013-1-1 |
| DOI | 10.2131/jts.38.503 |
| PII | DN/JST.JSTAGE/jts/38.503 |
| PMID | 23719928 |
| MeSH | Animals CHO Cells Cells, Cultured Contrast Media / toxicity* Cricetinae Cricetulus DNA Breaks, Double-Stranded / drug effects* Dose-Response Relationship, Drug Female Histones / metabolism Humans Magnetite Nanoparticles / toxicity* Micronuclei, Chromosome-Defective / drug effects* Phosphorylation Reactive Oxygen Species / metabolism* Sister Chromatid Exchange / drug effects* Time Factors |
| IF | 1.737 |
| Times Cited | 25 |
| WOS Category | TOXICOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | A549(RCB0098) CHO-AA8(RCB2326) |