論文 - 詳細
| RRC ID | 44460 |
|---|---|
| 著者 | Kawanishi M, Ogo S, Ikemoto M, Totsuka Y, Ishino K, Wakabayashi K, Yagi T. |
| タイトル | Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells. |
| ジャーナル | J Toxicol Sci |
| Abstract |
We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours' treatment with the particles dose-dependently increased the frequency of micronuclei (MN) in the A549 and CHO AA8 cells up to 5.2% and 5.0% at a dose of 200 µg/ml (34 µg/cm²), respectively. In A549 cells, treatment with the nano-particles (2 µg/ml) for 1 hr induced H2AX phosphorylation, which is suggestive of DNA double strand breaks (DSB). Treating CHO AA8 cells with 2 µg/ml (0.34 µg/cm²) magnetite for 1 hour resulted in a five times higher frequency of sister chromatid exchange (SCE) than the control level. We detected reactive oxygen species (ROS) in CHO cells treated with the particles. These findings indicate that magnetite nano-particles induce ROS in mammalian cells, leading to the direct or indirect induction of DSB, followed by clastogenic events including MN and SCE. |
| 巻・号 | 38(3) |
| ページ | 503-11 |
| 公開日 | 2013-1-1 |
| DOI | 10.2131/jts.38.503 |
| PII | DN/JST.JSTAGE/jts/38.503 |
| PMID | 23719928 |
| MeSH | Animals CHO Cells Cells, Cultured Contrast Media / toxicity* Cricetinae Cricetulus DNA Breaks, Double-Stranded / drug effects* Dose-Response Relationship, Drug Female Histones / metabolism Humans Magnetite Nanoparticles / toxicity* Micronuclei, Chromosome-Defective / drug effects* Phosphorylation Reactive Oxygen Species / metabolism* Sister Chromatid Exchange / drug effects* Time Factors |
| IF | 1.737 |
| 引用数 | 25 |
| WOS 分野 | TOXICOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) CHO-AA8(RCB2326) |