論文 - 詳細
| RRC ID | 44497 |
|---|---|
| 著者 | Chen DL, Zeng ZL, Yang J, Ren C, Wang DS, Wu WJ, Xu RH. |
| タイトル | L1cam promotes tumor progression and metastasis and is an independent unfavorable prognostic factor in gastric cancer. |
| ジャーナル | J Hematol Oncol |
| Abstract |
BACKGROUND:Previous reports have demonstrated that L1cam is aberrantly expressed in various tumors. The potential role of L1cam in the progression and metastasis of gastric cancer is still not clear and needs exploring. METHODS:Expression of L1cam was evaluated in gastric cancer tissues and cell lines by immunohistochemistry and Western blot. The relationship between L1cam expression and clinicopathological characteristics was analyzed. The effects of L1cam on cell proliferation, migration and invasion were investigated in gastric cancer cell lines both in vitro and in vivo. The impact of L1cam on PI3K/Akt pathway was also evaluated. RESULTS:L1cam was overexpressed in gastric cancer tissues and cell lines. L1cam expression was correlated with aggressive tumor phenotype and poor overall survival in gastric cancer patients. Ectopic expression of L1cam in gastric cell lines significantly promoted cell proliferation, migration and invasion whereas knockdown of L1cam inhibited cell proliferation, migration and invasion in vitro as well as tumorigenesis and metastasis in vivo. The low level of phosphorylated Akt in HGC27 cells was up-regulated after ectopic expression of L1cam, whereas the high level of phosphorylated Akt in SGC7901 cells was suppressed by knockdown of L1cam. Moreover, the migration and invasion promoted by L1cam overexpression in gastric cancer cells could be abolished by either application of LY294002 (a phosphoinositide-3-kinase inhibitor) or knockdown of endogenous Akt by small interfering RNA. CONCLUSIONS:Our study demonstrated that L1cam, overexpressed in gastric cancer and associated with poor prognosis, plays an important role in the progression and metastasis of gastric cancer. |
| 巻・号 | 6 |
| ページ | 43 |
| 公開日 | 2013-6-27 |
| DOI | 10.1186/1756-8722-6-43 |
| PII | 1756-8722-6-43 |
| PMID | 23806079 |
| PMC | PMC3717076 |
| MeSH | Animals Cell Line, Tumor Cell Movement Cell Proliferation Disease Progression Female Humans Male Mice Mice, Inbred BALB C Mice, Nude Neoplasm Metastasis Neural Cell Adhesion Molecule L1 / genetics Neural Cell Adhesion Molecule L1 / metabolism* Phosphatidylinositol 3-Kinases / genetics Phosphatidylinositol 3-Kinases / metabolism Prognosis Proto-Oncogene Proteins c-akt / genetics Proto-Oncogene Proteins c-akt / metabolism Stomach Neoplasms / genetics Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology* Transfection |
| IF | 11.059 |
| 引用数 | 36 |
| WOS 分野 | ONCOLOGY HEMATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HGC-27(RCB0500) |