RRC ID 44502
著者 Usui T, Okada M, Hara Y, Yamawaki H.
タイトル Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation.
ジャーナル Am J Physiol Heart Circ Physiol
Abstract Eukaryotic elongation factor 2 kinase (eEF2K) is a Ca2+/calmodulin-dependent protein kinase. We recently demonstrated that eEF2K protein increases in mesenteric artery from spontaneously hypertensive rats (SHR). Pathogenesis of hypertension is regulated in part by vascular inflammation. We tested the hypothesis whether eEF2K mediates vascular inflammatory responses and development of hypertension. In vascular endothelial cells, small interfering RNA (siRNA) against eEF2K inhibited induction of VCAM-1 and endothelial-selectin as well as monocyte adhesion by TNF-α (10 ng/ml). eEF2K siRNA inhibited phosphorylation of JNK and NF-κB p65 as well as reactive oxygen species (ROS) production by TNF-α. In vascular smooth muscle cells, eEF2K siRNA also inhibited VCAM-1 induction and phosphorylation of JNK and NF-κB by TNF-α. In vivo, increased blood pressure in SHR and ROS production, induction of inflammatory molecules, and hypertrophy in SHR superior mesenteric artery were reduced by an eEF2K inhibitor NH125 (500 μg·kg(-1)·day(-1)). In SHR superior mesenteric artery, impairment of ACh-induced relaxation was normalized by NH125. The present results for the first time demonstrate that eEF2K mediates TNF-α-induced vascular inflammation via ROS-dependent mechanism, which is at least partly responsible for the development of hypertension in SHR.
巻・号 305(5)
ページ H756-68
公開日 2013-9-1
DOI 10.1152/ajpheart.00373.2013
PII ajpheart.00373.2013
PMID 23812389
PMC PMC4073962
MeSH Animals Blood Pressure / drug effects Blood Pressure / physiology Cells, Cultured Disease Models, Animal Elongation Factor 2 Kinase / antagonists & inhibitors Elongation Factor 2 Kinase / drug effects Elongation Factor 2 Kinase / physiology* Endothelium, Vascular / drug effects Endothelium, Vascular / metabolism Endothelium, Vascular / pathology Humans Hypertension / metabolism Hypertension / physiopathology* Imidazoles / pharmacology MAP Kinase Kinase 4 / metabolism Male NF-kappa B / metabolism Oxidative Stress / physiology* RNA, Small Interfering / pharmacology Rats Rats, Inbred SHR Rats, Inbred WKY Rats, Wistar Reactive Oxygen Species / metabolism Tumor Necrosis Factor-alpha / adverse effects* Tumor Necrosis Factor-alpha / pharmacology Vascular Cell Adhesion Molecule-1 / metabolism Vasculitis / chemically induced* Vasculitis / metabolism Vasculitis / physiopathology*
IF 3.864
引用数 24
WOS 分野 PHYSIOLOGY CARDIAC & CARDIOVASCULAR SYSTEMS PERIPHERAL VASCULAR DISEASE
リソース情報
ヒト・動物細胞 U937