| RRC ID |
44827
|
| 著者 |
Seki Y, Yoshida Y, Ishimine H, Shinozaki-Ushiku A, Ito Y, Sumitomo K, Nakajima J, Fukayama M, Michiue T, Asashima M, Kurisaki A.
|
| タイトル |
Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Lung cancer is one of the most frequent causes of cancer-related death worldwide. However, molecular markers for lung cancer have not been well established. To identify novel genes related to lung cancer development, we surveyed publicly available DNA microarray data on lung cancer tissues. We identified lipase member H (LIPH, also known as mPA-PLA1) as one of the significantly upregulated genes in lung adenocarcinoma. LIPH was expressed in several adenocarcinoma cell lines when they were analyzed by quantitative real-time polymerase chain reaction (qPCR), western blotting, and sandwich enzyme-linked immunosorbent assay (ELISA). Immunohistochemical analysis detected LIPH expression in most of the adenocarcinomas and bronchioloalveolar carcinomas tissue sections obtained from lung cancer patients. LIPH expression was also observed less frequently in the squamous lung cancer tissue samples. Furthermore, LIPH protein was upregulated in the serum of early- and late-phase lung cancer patients when they were analyzed by ELISA. Interestingly, high serum level of LIPH was correlated with better survival in early phase lung cancer patients after surgery. Thus, LIPH may be a novel molecular biomarker for lung cancer, especially for adenocarcinoma and bronchioloalveolar carcinoma.
|
| 巻・号 |
443(4)
|
| ページ |
1141-7
|
| 公開日 |
2014-1-24
|
| DOI |
10.1016/j.bbrc.2013.12.106
|
| PII |
S0006-291X(13)02177-3
|
| PMID |
24380866
|
| MeSH |
Adenocarcinoma / genetics*
Adenocarcinoma / metabolism*
Adenocarcinoma / pathology
Adenocarcinoma of Lung
Adenocarcinoma, Bronchiolo-Alveolar / genetics*
Adenocarcinoma, Bronchiolo-Alveolar / metabolism*
Aged
Biomarkers, Tumor / blood
Biomarkers, Tumor / genetics*
Biomarkers, Tumor / metabolism*
Carcinoma, Non-Small-Cell Lung / genetics
Carcinoma, Non-Small-Cell Lung / metabolism
Carcinoma, Small Cell / genetics
Carcinoma, Small Cell / metabolism
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism
Cell Line, Tumor
Cell Proliferation
Female
Gene Knockdown Techniques
Humans
Lipase / blood
Lipase / genetics*
Lipase / metabolism*
Lung Neoplasms / genetics*
Lung Neoplasms / metabolism*
Lung Neoplasms / pathology
Male
Middle Aged
Prognosis
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Neoplasm / genetics
RNA, Neoplasm / metabolism
Up-Regulation
|
| IF |
2.985
|
| 引用数 |
10
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
LC-2/ad(RCB0440)
RERF-LC-KJ(RCB1313)
RERF-LC-AI(RCB0444)
EBC-1(RCB1965)
LK-2(RCB1970) |
