Reference - Detail
| RRC ID | 45180 |
|---|---|
| Author | Mino K, Nishimura S, Ninomiya S, Tujii H, Matsumori Y, Tsuchida M, Hosoi M, Koseki K, Wada S, Hasegawa M, Sasaki R, Murakami-Yamaguchi Y, Narita H, Suzuki T, Miyata N, Mizukami T. |
| Title | Regulation of tissue factor pathway inhibitor-2 (TFPI-2) expression by lysine-specific demethylase 1 and 2 (LSD1 and LSD2). |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Tissue factor pathway inhibitor-2 (TFPI-2) is a major inhibitor of extracellular matrix degradation. Decreases in TFPI-2 contribute to malignant tumor cell production, and TFPI-2 is a presumed tumor suppressor. TFPI-2 gene transcription is regulated by two epigenetic mechanisms: DNA methylation of the promoter and K4 methylation of histone 3 (H3). Lysine-specific demethylase 1 (LSD1) and LSD2 demethylate H3K4me2/1. LSD1 has been implicated in TFPI-2 regulation through both epigenetic mechanisms, but the involvement of LSD2 remains unknown. We prepared a monoclonal anti-LSD2 antibody that clearly distinguishes LSD2 from LSD1. Knockdown of LSD1 or LSD2 by siRNAs increased TFPI-2 protein and mRNA. Simultaneous knockdown of both LSD1 and LSD2 showed additive effects. Bisulfite sequencing revealed that CpG sites in the TFPI-2 promoter region were unmethylated. These results indicate that LSD2 also contributes to TFPI-2 regulation through histone modification, and that further studies of the involvement of LSD2 in tumor malignancy are warranted. |
| Volume | 78(6) |
| Pages | 1010-7 |
| Published | 2014-1-1 |
| DOI | 10.1080/09168451.2014.910104 |
| PMID | 25036127 |
| MeSH | Carcinogenesis DNA Methylation / drug effects Endodeoxyribonucleases Enzyme Inhibitors / pharmacology Gene Expression Regulation* / drug effects Gene Knockdown Techniques Glycoproteins / genetics* HEK293 Cells Histone Demethylases / antagonists & inhibitors Histone Demethylases / deficiency Histone Demethylases / genetics Histone Demethylases / metabolism* Histones / metabolism Humans Promoter Regions, Genetic / genetics |
| IF | 1.516 |
| Times Cited | 9 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | 293(RCB1637) |