論文 - 詳細
| RRC ID | 45548 |
|---|---|
| 著者 | D'Alessandro M, Hnia K, Gache V, Koch C, Gavriilidis C, Rodriguez D, Nicot AS, Romero NB, Schwab Y, Gomes E, Labouesse M, Laporte J. |
| タイトル | Amphiphysin 2 Orchestrates Nucleus Positioning and Shape by Linking the Nuclear Envelope to the Actin and Microtubule Cytoskeleton. |
| ジャーナル | Dev Cell |
| Abstract |
Nucleus positioning is key for intracellular organization, cell differentiation, and organ development and is affected in many diseases, including myopathies due to alteration in amphiphysin-2 (BIN1). The actin and microtubule cytoskeletons are essential for nucleus positioning, but their crosstalk in this process is sparsely characterized. Here, we report that impairment of amphiphysin/BIN1 in Caenorhabditis elegans, mammalian cells, or muscles from patients with centronuclear myopathy alters nuclear position and shape. We show that AMPH-1/BIN1 binds to nesprin and actin, as well as to the microtubule-binding protein CLIP170 in both species. Expression of the microtubule-anchoring CAP-GLY domain of CLIP170 fused to the nuclear-envelope-anchoring KASH domain of nesprin rescues nuclear positioning defects of amph-1 mutants. Amphiphysins thus play a central role in linking the nuclear envelope with the actin and microtubule cytoskeletons. We propose that BIN1 has a direct and evolutionarily conserved role in nuclear positioning, altered in myopathies. |
| 巻・号 | 35(2) |
| ページ | 186-98 |
| 公開日 | 2015-10-26 |
| DOI | 10.1016/j.devcel.2015.09.018 |
| PII | S1534-5807(15)00619-X |
| PMID | 26506308 |
| MeSH | Actins / genetics Animals COS Cells Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Cell Nucleus / genetics* Cell Shape / genetics Chlorocebus aethiops Cytoplasm / metabolism Cytoskeleton / genetics Cytoskeleton / metabolism Cytoskeleton / pathology HEK293 Cells Humans Microfilament Proteins / genetics* Microfilament Proteins / metabolism Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / metabolism Microtubules / genetics Microtubules / metabolism Multiprotein Complexes Muscle, Skeletal / metabolism Myopathies, Structural, Congenital / genetics* Myopathies, Structural, Congenital / metabolism Myopathies, Structural, Congenital / pathology Neoplasm Proteins / genetics* Neoplasm Proteins / metabolism Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Nuclear Envelope / genetics* Nuclear Envelope / metabolism Nuclear Proteins / genetics* Nuclear Proteins / metabolism |
| IF | 10.092 |
| 引用数 | 30 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1060 |