論文 - 詳細
| RRC ID | 45628 |
|---|---|
| 著者 | Prevo B, Mangeol P, Oswald F, Scholey JM, Peterman EJ. |
| タイトル | Functional differentiation of cooperating kinesin-2 motors orchestrates cargo import and transport in C. elegans cilia. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Intracellular transport depends on cooperation between distinct motor proteins. Two anterograde intraflagellar transport (IFT) motors, heterotrimeric kinesin-II and homodimeric OSM-3, cooperate to move cargo along Caenorhabditis elegans cilia. Here, using quantitative fluorescence microscopy, with single-molecule sensitivity, of IFT in living strains containing single-copy transgenes encoding fluorescent IFT proteins, we show that kinesin-II transports IFT trains through the ciliary base and transition zone to a 'handover zone' on the proximal axoneme. There, OSM-3 gradually replaces kinesin-II, yielding velocity profiles inconsistent with in vitro motility assays, and then drives transport to the ciliary tip. Dissociated kinesin-II motors undergo rapid turnaround and recycling to the ciliary base, whereas OSM-3 is recycled mainly to the handover zone. This reveals a functional differentiation in which the slower, less processive kinesin-II imports IFT trains into the cilium and OSM-3 drives their long-range transport, thereby optimizing cargo delivery. |
| 巻・号 | 17(12) |
| ページ | 1536-45 |
| 公開日 | 2015-12-1 |
| DOI | 10.1038/ncb3263 |
| PII | ncb3263 |
| PMID | 26523365 |
| MeSH | A Kinase Anchor Proteins / genetics A Kinase Anchor Proteins / metabolism Algorithms Animals Biological Transport / genetics Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cilia / metabolism* Computer Simulation Kinesins / chemistry Kinesins / genetics Kinesins / metabolism* Kinetics Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Fluorescence Models, Biological Mutation Protein Multimerization Video Recording |
| IF | 20.042 |
| 引用数 | 70 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3433 |