| RRC ID |
45718
|
| Author |
de Albuquerque BF, Luteijn MJ, Cordeiro Rodrigues RJ, van Bergeijk P, Waaijers S, Kaaij LJ, Klein H, Boxem M, Ketting RF.
|
| Title |
PID-1 is a novel factor that operates during 21U-RNA biogenesis in Caenorhabditis elegans.
|
| Journal |
Genes Dev
|
| Abstract |
The Piwi-piRNA pathway represents a small RNA-based mechanism responsible for the recognition and silencing of invading DNA. Biogenesis of piRNAs (21U-RNAs) is poorly understood. In Caenorhabditis elegans, the piRNA-binding Argonaute protein PRG-1 is the only known player acting downstream from precursor transcription. From a screen aimed at the isolation of piRNA-induced silencing-defective (Pid) mutations, we identified, among known Piwi pathway components, PID-1 as a novel player. PID-1 is a mostly cytoplasmic, germline-specific factor essential for 21U-RNA biogenesis, affecting an early step in the processing or transport of 21U precursor transcripts. We also show that maternal 21U-RNAs are essential to initiate silencing.
|
| Volume |
28(7)
|
| Pages |
683-8
|
| Published |
2014-4-1
|
| DOI |
10.1101/gad.238220.114
|
| PII |
28/7/683
|
| PMID |
24696453
|
| PMC |
PMC4015495
|
| MeSH |
Animals
Argonaute Proteins / genetics
Argonaute Proteins / metabolism
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism*
Methylation
Mutation
Protein Precursors / metabolism
RNA Interference / physiology
RNA, Small Interfering / biosynthesis*
Transgenes / genetics
|
| IF |
9.527
|
| Times Cited |
14
|
|
WOS Category
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm1200 |
