RRC ID 45725
著者 Edmonds MJ, Morgan A.
タイトル A systematic analysis of protein palmitoylation in Caenorhabditis elegans.
ジャーナル BMC Genomics
Abstract BACKGROUND:Palmitoylation is a reversible post-translational protein modification which involves the addition of palmitate to cysteine residues. Palmitoylation is catalysed by the DHHC family of palmitoyl-acyl transferases (PATs) and reversibility is conferred by palmitoyl-protein thioesterases (PPTs). Mutations in genes encoding both classes of enzymes are associated with human diseases, notably neurological disorders, underlining their importance. Despite the pivotal role of yeast studies in discovering PATs, palmitoylation has not been studied in the key animal model Caenorhabditis elegans.
RESULTS:Analysis of the C. elegans genome identified fifteen PATs, using the DHHC cysteine-rich domain, and two PPTs, by homology. The twelve uncategorised PATs were officially named using a dhhc-x system. Genomic data on these palmitoylation enzymes and those in yeast, Drosophila and humans was collated and analysed to predict properties and relationships in C. elegans. All available C. elegans strains containing a mutation in a palmitoylation enzyme were analysed and a complete library of RNA interference (RNAi) feeding plasmids against PAT or PPT genes was generated. To test for possible redundancy, double RNAi was performed against selected closely related PATs and both PPTs. Animals were screened for phenotypes including size, longevity and sensory and motor neuronal functions. Although some significant differences were observed with individual mutants or RNAi treatment, in general there was little impact on these phenotypes, suggesting that genetic buffering exists within the palmitoylation network in worms.
CONCLUSIONS:This study reports the first characterisation of palmitoylation in C. elegans using both in silico and in vivo approaches, and opens up this key model organism for further detailed study of palmitoylation in future.
巻・号 15(1)
ページ 841
公開日 2014-10-2
DOI 10.1186/1471-2164-15-841
PII 1471-2164-15-841
PMID 25277130
PMC PMC4192757
MeSH Acyltransferases / chemistry Acyltransferases / deficiency Acyltransferases / genetics Acyltransferases / metabolism Aging / genetics Aging / metabolism Aging / physiology Amino Acid Sequence Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / metabolism* Computational Biology* Gene Knockdown Techniques Humans Lipoylation* Locomotion / genetics Molecular Sequence Data Mutation Phenotype Protein Processing, Post-Translational* RNA Interference Sequence Analysis
IF 3.594
引用数 11
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
線虫 tm4272