Reference - Detail
| RRC ID | 45811 |
|---|---|
| Author | Murayama Y, Ogura T, Yamanaka K. |
| Title | Characterization of C-terminal adaptors, UFD-2 and UFD-3, of CDC-48 on the polyglutamine aggregation in C. elegans. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
CDC-48 (also called VCP or p97 in mammals and Cdc48p in yeast) is a AAA (ATPases associated with diverse cellular activities) chaperone and participates in a wide range of cellular activities including modulation of protein complexes and protein aggregates. UFD-2 and UFD-3, C-terminal adaptors for CDC-48, reportedly bind to CDC-48 in a mutually exclusive manner and they may modulate the fate of substrates for CDC-48. However, their cellular functions have not yet been elucidated. In this study, we found that CDC-48 preferentially interacts with UFD-3 in Caenorhabditis elegans. We also found that the number of polyglutamine (polyQ) aggregates was reduced in the ufd-3 deletion mutant but not in the ufd-2 deletion mutant. Furthermore, the lifespan and motility of the ufd-3 deletion mutant, where polyQ40::GFP was expressed, were greatly decreased. Taken together, we propose that UFD-3 may promote the formation of polyQ aggregates to reduce the polyQ toxicity in C. elegans. |
| Volume | 459(1) |
| Pages | 154-60 |
| Published | 2015-3-27 |
| DOI | 10.1016/j.bbrc.2015.02.088 |
| PII | S0006-291X(15)00316-2 |
| PMID | 25721663 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Aging / genetics Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Gene Deletion Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Mutation Ubiquitin-Protein Ligase Complexes / genetics Ubiquitin-Protein Ligase Complexes / metabolism* Valosin Containing Protein |
| IF | 2.985 |
| Times Cited | 4 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| C.elegans | tm544 tm1380 tm2915 |