Reference - Detail
| RRC ID | 45881 |
|---|---|
| Author | Weinberg F, Schulze E, Fatouros C, Schmidt E, Baumeister R, Brummer T. |
| Title | Expression pattern and first functional characterization of riok-1 in Caenorhabditis elegans. |
| Journal | Gene Expr Patterns |
| Abstract |
Rio kinases are atypical serine/threonine kinases that emerge as potential cooperation partners in Ras-driven tumors. In the current study, we performed an RNAi screen in Caenorhabditis elegans to identify suppressors of oncogenic Ras signaling. Aberrant Ras/Raf signaling in C. elegans leads to the formation of a multi-vulva (Muv) phenotype. We found that depletion of riok-1, the C. elegans orthologue of the mammalian RioK1, suppressed the Muv phenotype. By using a promoter GFP construct, we could show that riok-1 is expressed in neuronal cells, the somatic gonad, the vulva, the uterus and the spermatheca. Furthermore, we observed developmental defects in the gonad upon riok-1 knockdown in a wildtype background. Our data suggest that riok-1 is a modulator of the Ras signaling pathway, suggesting implications for novel interventions in the context of Ras-driven tumors. |
| Volume | 15(2) |
| Pages | 124-34 |
| Published | 2014-7-1 |
| DOI | 10.1016/j.gep.2014.05.005 |
| PII | S1567-133X(14)00053-2 |
| PMID | 24929033 |
| MeSH | Animals Base Sequence Butadienes / chemistry Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / metabolism* Cell Lineage Enzyme Inhibitors / chemistry Extracellular Signal-Regulated MAP Kinases / metabolism Gene Expression Profiling* Gene Expression Regulation, Developmental* Gene Expression Regulation, Enzymologic* Gonads / embryology Molecular Sequence Data Neurons / metabolism Nitriles / chemistry Phenotype Protein Serine-Threonine Kinases / metabolism* RNA Interference Signal Transduction Time Factors |
| IF | 0.897 |
| Times Cited | 8 |
| WOS Category | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY |
| Resource | |
| C.elegans | tm3775 |